The protein staining procedure becomes more cost-efficient with ProteOrange dye, which can be used for protein staining in fixed cells, as a good example. From a structural viewpoint, ProteOrange Protein Gel Stain is an equivalent of the already well-known SYPRO™ Orange Protein Gel Stain. Below shown image demonstrates the ability of ProteOrange to stain the proteins in the cytoplasm after 30 min stirring with the dye.
Briefly about the protein staining procedure on fixed cells: the cells were fixed using formaldehyde and then washed a couple of times with 1X PBS buffer. 1 uM ProteOrange dye solution in PBS was added to fixed cells and stirred for 30 mins at room temperature. After washing a couple of times with 1X PBS buffer to remove free ProteOrange dye, cells were visualized using fluorescence microscopy. The nuclei were stained using Hoechst dye. The resulting image is shown below:
Staining of fixed cells with ProteOrange® dye.
Staining was performed for 30 min in 1 uM dye solution.