Decreased labeling efficiency of CuAAC and SPAAC click reactions due to azide presence in reaction buffer.
Updated 3 months ago
Click chemistry
Click chemistry related questions
Does azide ion interfere with click chemistry reactions (CuAAC and SPAAC)?
Does nonspecific protein labeling exist in click chemistry reactions?
A comparison of nonspecific interactions in different click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted alkyne-azide cycloaddition (SPAAC).
Updated 1 year ago