Customers' Q&A

Categories
Customers' Q&A

How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?

Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen for DNA detection by gel soaking.

Updated 4 hours ago

Does the fluorescence of cyanine dyes depend on pH?

The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.

Updated 2 months ago

What is hydrolysis rates for NHS Ester functionalized dyes?

NHS ester tends to hydrolyze over the time in water.

Updated 4 months ago

Is it possible to use Pico488 quantification solution or kit with the most popular reader?

Short description how to replace the dsDNA HS Assay Kit by the Pico488 kit/reagent.

Updated 5 months ago

How to perform the measurements with fluorometer using QuDye Protein Quantification kit?

In this article is in details described, how to take the measurements with fluorometer using QuDye Protein Quantification kit.

Updated 5 months ago

What is the best practice of Electrophoresis gel staining with dsGreen?

In this article we have compared different methods of using dsGreen gel staining dye to indicate difference between EtBr and DsGreen.

Updated 6 months ago

Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?

Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).

Updated 7 months ago

How to get a fluorescent derivative of biomolecule using dye hydrazide?

This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.

Updated 7 months ago

What is the solubility of BDP FL Hydrazide in water?

Water solubility of BDP FL Hydrazide dye in water

Updated 7 months ago

Does nonspecific protein labeling exist in Click Chemistry reactions?

A comparison of nonspecific interactions in different Click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted alkyne-azide cycloaddition (spAAC).

Updated 7 months ago

Does azide ion interfere with Click chemistry reactions (CuAAC and spAAC)?

Decreased labeling efficiency of CuAAC and spAAC click reactions due to azide presence in reaction buffer.

Updated 11 months ago

Conjugation of antibodies with DBCO and downstream copper free Click chemistry reaction

Copper free Click Chemistry conjugation guidelines, considerations, reaction scheme.

Updated 11 months ago

Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.

Updated 11 months ago

A guide for visualization of gels stained with dsGreen using Gel Doc™ EZ System.

Detailed step-by-step guidance on how to use Gel Doc™ EZ System by Bio-Rad for visualization of gels stained with dsGreen.

Updated 11 months ago

Do you have a protocol for labeling of proteins with hydrazide-based dyes?

Brief protocol for protein labeling using hydrazide funtionalized dyes.

Updated 1 year ago

How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments.

Updated 1 year ago

How to attach DBCO (ADIBO) group to an antibody?

Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.

Updated 1 year ago

How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?

Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.

Updated 1 year ago

Staining lipid droplets with BDP 493/503 and BDP 505/515

General recommendations for staining of lipid droplets with BDP493/503 using HepG2 culture as a model

Updated 1 year ago

Why Cyanine dyes have low quantum yields, but still widely used?

Quantum yield of Cyanine dyes

Updated 2 years ago

I’ve been using your cyanine7.5 amine to label some of my polymers but I can’t seem to get any signal when I test the fluorescence on the fluorometer.

Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.

Updated 2 years ago

We attempted to attach Sulfo-Cy7-NHS ester to modified triphosphate, but the resultant product had really low fluorescence.

Low fluorescence of Sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.

Updated 2 years ago

What is the solubility of Cyanine7.5 amine in water?

Water solubility of Cyanine7.5 amine product.

Updated 2 years ago

Are Cyanine dyes stable in 0.1-0.5% bleach solution?

Stability of Cyanine dyes in bleach solution

Updated 3 years ago

Staining proteins with ProteOrange in polyacrylamide gels.

Step-by-step instruction on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.

Updated 3 years ago

What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?

Water solubility of Cy7 amine derivatives

Updated 3 years ago

I would like to label tyramine molecule with fluorescent dye, is it possible?

The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.

Updated 3 years ago

BDP 581/591 dye for detection and monitoring of cellular oxidative stress

BDP 581/591 dye for detection of reactive oxygen species (ROS) in cultured mammalian cells by flow cytometry

Updated 3 years ago

Can I stain proteins in fixed cells with ProteOrange dye?

Short description of Protein staining experience with ProteOrange dye from Lumiprobe.

Updated 3 years ago

Is it possible to label dilute antibody with the ready kit?

Even when the concentration of Ab in the solution is below the recommended concentraiton of 1 mg/mL, it is still possible to use Lumiprobe antibody labeling kit to achieve labeling degree of 2-3 dye molecules per Ab.

Updated 3 years ago

Is there any concern on a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo. Does fluorescence intensity remain or get weak by epoxidation?

Epoxidation of Cyanine dyes

Updated 4 years ago

Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?

Specificity of Pico488 for binding dsDNA versus ssDNA.

Updated 4 years ago

What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?

The solubility of Cyanine7 derivatives in different organic solvents.

Updated 4 years ago

Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?

Non-sulfonated versus sulfonated dyes for proteins labeling with our dyes.