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Customers' Q&A

All interesting content we have reported to our customers following their requests.

Alexander FomenkovNikita OskolkovMarina Goryunova

32 articles in this category / Updated 5 days ago

Written by 3 authors

What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?
What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?

Water solubility of Cy7 amine derivatives

Updated 5 days ago

How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?
How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?

Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen or SYBR Green for DNA detection by gel soaking.

Updated 15 days ago

Do you have a protocol for labeling of proteins with hydrazide-based dyes?
Do you have a protocol for labeling of proteins with hydrazide-based dyes?

Brief protocol for protein labeling using hydrazide funtionalized dyes.

Updated 23 days ago

BDP 581/591 dye for detection and monitoring of cellular oxidative stress
BDP 581/591 dye for detection and monitoring of cellular oxidative stress

BDP 581/591 dye for detection of reactive oxygen species (ROS) in cultured mammalian cells by flow cytometry

Updated 1 month ago

Quantitative real-time PCR with dsGreen?
Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.

Updated 2 months ago

Does nonspecific protein labeling exist in Click Chemistry reactions?
Does nonspecific protein labeling exist in Click Chemistry reactions?

A comparison of nonspecific interactions in different Click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted alkyne-azide cycloaddition (spAAC).

Updated 2 months ago

How to get a fluorescent derivative of biomolecule using dye hydrazide?
How to get a fluorescent derivative of biomolecule using dye hydrazide?

This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.

Updated 3 months ago

Does the fluorescence of cyanine dyes depend on pH?
Does the fluorescence of cyanine dyes depend on pH?

The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.

Updated 3 months ago

Can I stain proteins in fixed cells with ProteOrange dye?
Can I stain proteins in fixed cells with ProteOrange dye?

Short description of Protein staining experience with ProteOrange dye from Lumiprobe.

Updated 3 months ago

Labeling of biomolecules with DBCO SE and dye azide. What is necessary for DBCO-based conjugation reaction?
Labeling of biomolecules with DBCO SE and dye azide. What is necessary for DBCO-based conjugation reaction?

Copper free Click Chemistry conjugation guidelines, considerations, reaction scheme.

Updated 4 months ago

Is it possible to label dilute antibody with the ready kit?
Is it possible to label dilute antibody with the ready kit?

Even when the concentration of Ab in the solution is below the recommended concentraiton of 1 mg/mL, it is still possible to use Lumiprobe antibody labeling kit to achieve labeling degree of 2-3 dye molecules per Ab.

Updated 4 months ago

How to perform the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit?
How to perform the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit?

In this article is in details described, how to take the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit.

Updated 4 months ago

How to attach DBCO (ADIBO) group to an antibody?
How to attach DBCO (ADIBO) group to an antibody?

Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.

Updated 6 months ago

Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?

Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).

Updated 7 months ago

Is there any concern on a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo. Does fluorescence intensity remain or get weak by epoxidation?
Is there any concern on a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo. Does fluorescence intensity remain or get weak by epoxidation?

Epoxidation of Cyanine dyes

Updated 7 months ago

Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?
Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?

Specificity of Pico488 for binding dsDNA versus ssDNA.

Updated 8 months ago

What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?
What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?

The solubility of Cyanine7 derivatives in different organic solvents.

Updated 8 months ago

Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?

Non-sulfonated versus sulfonated dyes for proteins labeling with our dyes.

Updated 9 months ago

What does "minimal" mean in Cyanine NHS ester minimal dye products and can Cyanine2, Cyanine3 and Cyanine5 be mixed all together?
What does "minimal" mean in Cyanine NHS ester minimal dye products and can Cyanine2, Cyanine3 and Cyanine5 be mixed all together?

"Minimal" meaning in Cyanine NHS ester minimal dye products for DIGE applications

Updated 9 months ago

How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?
How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?

Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit

Updated 10 months ago

Is it possible to use Pico488 quantification solution or kit with Qubit™ reader?
Is it possible to use Pico488 quantification solution or kit with Qubit™ reader?

Short description how to replace the Qubit™ dsDNA HS Assay Kit by the Pico488 kit/reagent.

Updated 11 months ago

What is the structure of the dye in Pico488 DNA quantification solution?
What is the structure of the dye in Pico488 DNA quantification solution?

The chemical structure for dye used in Pico488 solution.

Updated 11 months ago

What is the best practice of Electrophoresis gel staining with dsGreen?
What is the best practice of Electrophoresis gel staining with dsGreen?

In this article we have compared different methods of using dsGreen gel staining dye to indicate difference between EtBr and DsGreen.

Updated 11 months ago

How to use passive PCR reference dye?
How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments.

Updated 11 months ago

Why Cyanine dyes have low quantum yields, but still widely used?
Why Cyanine dyes have low quantum yields, but still widely used?

Quantum yield of Cyanine dyes

Updated 11 months ago

How do I calculate the optimal amounts and procedure to label protein/antibody?
How do I calculate the optimal amounts and procedure to label protein/antibody?

This article as about fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.

Updated 11 months ago

Is it possible to label Tyrosine containing peptides?
Is it possible to label Tyrosine containing peptides?

Labeling of the Tyr containing peptides is possible according to literature.

Updated 11 months ago

Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?
Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?

Using distilled water for maleimide labeling

Updated 11 months ago

What is the proper color of Cyanine5 SE - succinimidyl ester?
What is the proper color of Cyanine5 SE - succinimidyl ester?

This article is to explain possible appearance and colours range you may observe for Cyanine 5 NHS ester powder/thin films.

Updated 11 months ago

Is NIR dye visible in the day light?
Is NIR dye visible in the day light?

Is there any visible colour change when excited?

Updated 11 months ago

How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?
How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?

Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.

Updated 11 months ago

What is hydrolysis rates for NHS Ester functionalized dyes?
What is hydrolysis rates for NHS Ester functionalized dyes?

NHS ester tends to hydrolyze over the time in water.

Updated 11 months ago