In this article we have compared different methods of using dsGreen gel staining dye to indicate difference between EtBr and DsGreen.
Updated diff.ago.day
Customers' Q&A
All interesting content we have reported to our customers following their requests.
What is the best practice of Electrophoresis gel staining with dsGreen?
How to perform the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit?
In this article is in details described, how to take the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit.
Updated diff.ago.day
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).
Updated diff.ago.month
How to attach DBCO (ADIBO) group to an antibody?
Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.
Updated diff.ago.month
Conjugation of antibodies with DBCO and downstream copper free Click chemistry reaction
Copper free Click Chemistry conjugation guidelines, considerations, reaction scheme.
Updated diff.ago.month
How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?
Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.
Updated diff.ago.month
Staining lipid droplets with BDP 493/503 and BDP 505/515
General recommendations for staining of lipid droplets with BDP493/503 using HepG2 culture as a model
Updated diff.ago.month
Why Cyanine dyes have low quantum yields, but still widely used?
Quantum yield of Cyanine dyes
Updated diff.ago.month
Does nonspecific protein labeling exist in Click Chemistry reactions?
A comparison of nonspecific interactions in different Click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted alkyne-azide cycloaddition (spAAC).
Updated diff.ago.month
I’ve been using your cyanine7.5 amine to label some of my polymers but I can’t seem to get any signal when I test the fluorescence on the fluorometer.
Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.
Updated diff.ago.month
Does azide ion interfere with Click chemistry reactions (CuAAC and spAAC)?
Decreased labeling efficiency of CuAAC and spAAC click reactions due to azide presence in reaction buffer.
Updated diff.ago.year
We attempted to attach Sulfo-Cy7-NHS ester to modified triphosphate, but the resultant product had really low fluorescence.
Low fluorescence of Sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.
Updated diff.ago.year
What is the solubility of Cyanine7.5 amine in water?
Water solubility of Cyanine7.5 amine product.
Updated diff.ago.year
What is the solubility of BDP FL Hydrazide in water?
Water solubility of BDP FL Hydrazide dye in water
Updated diff.ago.year
Does the fluorescence of cyanine dyes depend on pH?
The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.
Updated diff.ago.year
Are Cyanine dyes stable in 0.1-0.5% bleach solution?
Stability of Cyanine dyes in bleach solution
Updated diff.ago.year
Staining proteins with ProteOrange in polyacrylamide gels.
Step-by-step instruction on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.
Updated diff.ago.year
Do you have a protocol for labeling of proteins with hydrazide-based dyes?
Brief protocol for protein labeling using hydrazide funtionalized dyes.
Updated diff.ago.year
What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?
Water solubility of Cy7 amine derivatives
Updated diff.ago.year
A guide for visualization of gels stained with dsGreen using Gel Doc™ EZ System.
Detailed step-by-step guidance on how to use Gel Doc™ EZ System by Bio-Rad for visualization of gels stained with dsGreen or SYBR® Green.
Updated diff.ago.year
I would like to label tyramine molecule with fluorescent dye, is it possible?
The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.
Updated diff.ago.year
BDP 581/591 dye for detection and monitoring of cellular oxidative stress
BDP 581/591 dye for detection of reactive oxygen species (ROS) in cultured mammalian cells by flow cytometry
Updated diff.ago.year
How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?
Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen or SYBR Green for DNA detection by gel soaking.
Updated diff.ago.year
Quantitative real-time PCR with dsGreen?
Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.
Updated diff.ago.year
How to get a fluorescent derivative of biomolecule using dye hydrazide?
This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.
Updated diff.ago.year
Can I stain proteins in fixed cells with ProteOrange dye?
Short description of Protein staining experience with ProteOrange dye from Lumiprobe.
Updated diff.ago.year
Is it possible to label dilute antibody with the ready kit?
Even when the concentration of Ab in the solution is below the recommended concentraiton of 1 mg/mL, it is still possible to use Lumiprobe antibody labeling kit to achieve labeling degree of 2-3 dye molecules per Ab.
Updated diff.ago.year
Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?
Specificity of Pico488 for binding dsDNA versus ssDNA.
Updated diff.ago.year
What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?
The solubility of Cyanine7 derivatives in different organic solvents.
Updated diff.ago.year
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Non-sulfonated versus sulfonated dyes for proteins labeling with our dyes.
Updated diff.ago.year
What does "minimal" mean in Cyanine NHS ester minimal dye products and can Cyanine2, Cyanine3 and Cyanine5 be mixed all together?
"Minimal" meaning in Cyanine NHS ester minimal dye products for DIGE applications
Updated diff.ago.year
How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?
Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit
Updated diff.ago.year
Is it possible to use Pico488 quantification solution or kit with Qubit™ reader?
Short description how to replace the Qubit™ dsDNA HS Assay Kit by the Pico488 kit/reagent.
Updated diff.ago.year
What is the structure of the dye in Pico488 DNA quantification solution?
The chemical structure for dye used in Pico488 solution.
Updated diff.ago.year
How to use passive PCR reference dye?
This is a short description of the best practice to use ROX ref. dye in PCR experiments.
Updated diff.ago.year
How do I calculate the optimal amounts and procedure to label protein/antibody?
This article as about fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.
Updated diff.ago.year
Is it possible to label Tyrosine containing peptides?
Labeling of the Tyr containing peptides is possible according to literature.
Updated diff.ago.year
Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?
Using distilled water for maleimide labeling
Updated diff.ago.year
What is the proper color of Cyanine5 SE - succinimidyl ester?
This article is to explain possible appearance and colours range you may observe for Cyanine 5 NHS ester powder/thin films.
Updated diff.ago.year
Is NIR dye visible in the day light?
Is there any visible colour change when excited?
Updated diff.ago.year
What is hydrolysis rates for NHS Ester functionalized dyes?
NHS ester tends to hydrolyze over the time in water.
Updated diff.ago.year