Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen for DNA detection by gel soaking.
Updated 2 days ago
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Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen for DNA detection by gel soaking.
Updated 2 days ago
Copper free Click Chemistry conjugation guidelines, considerations, reaction scheme.
Updated 10 days ago
BDP 581/591 dye for detection of reactive oxygen species (ROS) in cultured mammalian cells by flow cytometry
Updated 13 days ago
A comparison of nonspecific interactions in different Click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted alkyne-azide cycloaddition (spAAC).
Updated 21 days ago
Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.
Updated 2 months ago
In this article is in details described, how to take the measurements with fluorometer using QuDye Protein Quantification kit.
Updated 2 months ago
The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.
Updated 6 months ago
NHS ester tends to hydrolyze over the time in water.
Updated 8 months ago
Short description how to replace the dsDNA HS Assay Kit by the Pico488 kit/reagent.
Updated 9 months ago
In this article we have compared different methods of using dsGreen gel staining dye to indicate difference between EtBr and DsGreen.
Updated 11 months ago
Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).
Updated 11 months ago
This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.
Updated 11 months ago
Water solubility of BDP FL Hydrazide dye in water
Updated 11 months ago
Decreased labeling efficiency of CuAAC and spAAC click reactions due to azide presence in reaction buffer.
Updated 1 year ago
Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.
Updated 1 year ago
Detailed step-by-step guidance on how to use Gel Doc™ EZ System by Bio-Rad for visualization of gels stained with dsGreen.
Updated 1 year ago
Brief protocol for protein labeling using hydrazide funtionalized dyes.
Updated 1 year ago
This is a short description of the best practice to use ROX ref. dye in PCR experiments.
Updated 1 year ago
Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.
Updated 1 year ago
General recommendations for staining of lipid droplets with BDP493/503 using HepG2 culture as a model
Updated 2 years ago
Quantum yield of Cyanine dyes
Updated 2 years ago
Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.
Updated 2 years ago
Low fluorescence of Sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.
Updated 3 years ago
Water solubility of Cyanine7.5 amine product.
Updated 3 years ago
Stability of Cyanine dyes in bleach solution
Updated 3 years ago
Step-by-step instruction on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.
Updated 3 years ago
Water solubility of Cy7 amine derivatives
Updated 3 years ago
The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.
Updated 3 years ago
Short description of Protein staining experience with ProteOrange dye from Lumiprobe.
Updated 4 years ago
Even when the concentration of Ab in the solution is below the recommended concentraiton of 1 mg/mL, it is still possible to use Lumiprobe antibody labeling kit to achieve labeling degree of 2-3 dye molecules per Ab.
Updated 4 years ago
Specificity of Pico488 for binding dsDNA versus ssDNA.
Updated 4 years ago
The solubility of Cyanine7 derivatives in different organic solvents.
Updated 4 years ago
Non-sulfonated versus sulfonated dyes for proteins labeling with our dyes.
Updated 4 years ago
"Minimal" meaning in Cyanine NHS ester minimal dye products for DIGE applications
Updated 4 years ago
Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit
Updated 4 years ago
The chemical structure for dye used in Pico488 solution.
Updated 4 years ago
This article as about fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.
Updated 4 years ago
Labeling of the Tyr containing peptides is possible according to literature.
Updated 4 years ago
Using distilled water for maleimide labeling
Updated 4 years ago
This article is to explain possible appearance and colours range you may observe for Cyanine 5 NHS ester powder/thin films.
Updated 4 years ago
Is there any visible colour change when excited?
Updated 4 years ago