Customers' Q&A

All interesting content we have reported to our customers following their requests.

Nikita OskolkovAlexander FomenkovMarina Goryunova

20 articles in this category / Updated 1 month ago

Written by 3 authors

We attempted to attach Sulfo-Cy7-NHS ester to modified triphosphate, but the resultant product had really low fluorescence.
We attempted to attach Sulfo-Cy7-NHS ester to modified triphosphate, but the resultant product had really low fluorescence.

Low fluorescence of Sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.

Updated 1 month ago

I would like to label tyramine molecule with fluorescent dye, is it possible?
I would like to label tyramine molecule with fluorescent dye, is it possible?

The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.

Updated 2 months ago

How to perform the measurements with fluorometer using QuDye Protein Quantification kit?
How to perform the measurements with fluorometer using QuDye Protein Quantification kit?

In this article is in details described, how to take the measurements with fluorometer using QuDye Protein Quantification kit.

Updated 6 months ago

Does the fluorescence of cyanine dyes depend on pH?
Does the fluorescence of cyanine dyes depend on pH?

The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.

Updated 10 months ago

What is hydrolysis rates for NHS Ester functionalized dyes?
What is hydrolysis rates for NHS Ester functionalized dyes?

NHS ester tends to hydrolyze over the time in water.

Updated 1 year ago

Is it possible to use Pico488 quantification solution or kit with the most popular reader?
Is it possible to use Pico488 quantification solution or kit with the most popular reader?

Short description how to replace the dsDNA HS Assay Kit by the Pico488 kit/reagent.

Updated 1 year ago

Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?

Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).

Updated 1 year ago

What is the solubility of BDP FL Hydrazide in water?
What is the solubility of BDP FL Hydrazide in water?

Water solubility of BDP FL Hydrazide dye in water

Updated 1 year ago

Why Cyanine dyes have low quantum yields, but still widely used?
Why Cyanine dyes have low quantum yields, but still widely used?

Quantum yield of Cyanine dyes

Updated 2 years ago

I’ve been using your cyanine7.5 amine to label some of my polymers but I can’t seem to get any signal when I test the fluorescence on the fluorometer.
I’ve been using your cyanine7.5 amine to label some of my polymers but I can’t seem to get any signal when I test the fluorescence on the fluorometer.

Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.

Updated 3 years ago

What is the solubility of Cyanine7.5 amine in water?
What is the solubility of Cyanine7.5 amine in water?

Water solubility of Cyanine7.5 amine product.

Updated 3 years ago

Are Cyanine dyes stable in 0.1-0.5% bleach solution?
Are Cyanine dyes stable in 0.1-0.5% bleach solution?

Stability of Cyanine dyes in bleach solution

Updated 3 years ago

What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?
What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?

Water solubility of Cy7 amine derivatives

Updated 3 years ago

Is there any concern on a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo. Does fluorescence intensity remain or get weak by epoxidation?
Is there any concern on a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo. Does fluorescence intensity remain or get weak by epoxidation?

Epoxidation of Cyanine dyes

Updated 4 years ago

Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?
Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?

Specificity of Pico488 for binding dsDNA versus ssDNA.

Updated 4 years ago

What is the solubility of  Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?
What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?

The solubility of Cyanine7 derivatives in different organic solvents.

Updated 4 years ago

What is the structure of the dye in Pico488 DNA quantification solution?
What is the structure of the dye in Pico488 DNA quantification solution?

The chemical structure for dye used in Pico488 solution.

Updated 4 years ago

Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?
Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?

Using distilled water for maleimide labeling

Updated 5 years ago

What is the proper color of Cyanine5 SE - succinimidyl ester?
What is the proper color of Cyanine5 SE - succinimidyl ester?

This article is to explain possible appearance and colours range you may observe for Cyanine 5 NHS ester powder/thin films.

Updated 5 years ago

Is NIR dye visible in the day light?
Is NIR dye visible in the day light?

Is there any visible colour change when excited?

Updated 5 years ago