A comparison of nonspecific interactions in different Click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain promoted alkyne-azide cycloaddition (spAAC).
Updated 1 month ago
Customers' Q&A
All interesting content we have reported to our customers following their requests.
Does nonspecific protein labeling exist in Click Chemistry reactions?
I’ve been using your cyanine7.5 amine to label some of my polymers but I can’t seem to get any signal when I test the fluorescence on the fluorometer.
Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.
Updated 3 months ago
Does azide ion interfere with Click chemistry reactions (CuAAC and spAAC)?
Decreased labeling efficiency of CuAAC and spAAC click reactions due to azide presence in reaction buffer.
Updated 5 months ago
Conjugation of antibodies with DBCO and downstream copper free Click chemistry reaction
Copper free Click Chemistry conjugation guidelines, considerations, reaction scheme.
Updated 6 months ago
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).
Updated 6 months ago
We attempted to attach Sulfo-Cy7-NHS ester to modified triphosphate, but the resultant product had really low fluorescence.
Low fluorescence of Sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.
Updated 6 months ago
What is the solubility of Cyanine7.5 amine in water?
Water solubility of Cyanine7.5 amine product.
Updated 9 months ago
What is the solubility of BDP FL Hydrazide in water?
Water solubility of BDP FL Hydrazide dye in water
Updated 10 months ago
Does the fluorescence of cyanine dyes depend on pH?
The fluorescence dependency of cyanine dyes such as Cyanine3, Sulfo-Cyanine3, Cyanine5, Sulfo-Cyanine5 and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.
Updated 10 months ago
Are Cyanine dyes stable in 0.1-0.5% bleach solution?
Stability of Cyanine dyes in bleach solution
Updated 11 months ago
Staining proteins with ProteOrange in polyacrylamide gels.
Step-by-step instruction on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.
Updated 11 months ago
Do you have a protocol for labeling of proteins with hydrazide-based dyes?
Brief protocol for protein labeling using hydrazide funtionalized dyes.
Updated 1 year ago
What is solubility of Sulfo-Cyanine7 amine and Cyanine7 amine in water?
Water solubility of Cy7 amine derivatives
Updated 1 year ago
A guide for visualization of gels stained with dsGreen using Gel Doc™ EZ System.
Detailed step-by-step guidance on how to use Gel Doc™ EZ System by Bio-Rad for visualization of gels stained with dsGreen or SYBR® Green.
Updated 1 year ago
Why Cyanine dyes have low quantum yields, but still widely used?
Quantum yield of Cyanine dyes
Updated 1 year ago
I would like to label tyramine molecule with fluorescent dye, is it possible?
The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.
Updated 1 year ago
BDP 581/591 dye for detection and monitoring of cellular oxidative stress
BDP 581/591 dye for detection of reactive oxygen species (ROS) in cultured mammalian cells by flow cytometry
Updated 1 year ago
How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?
Step By Step description how to prepare Agarose gel and to use dsDNA binding dye like dsGreen or SYBR Green for DNA detection by gel soaking.
Updated 1 year ago
Quantitative real-time PCR with dsGreen?
Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.
Updated 1 year ago
How to get a fluorescent derivative of biomolecule using dye hydrazide?
This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.
Updated 1 year ago
Can I stain proteins in fixed cells with ProteOrange dye?
Short description of Protein staining experience with ProteOrange dye from Lumiprobe.
Updated 1 year ago
Is it possible to label dilute antibody with the ready kit?
Even when the concentration of Ab in the solution is below the recommended concentraiton of 1 mg/mL, it is still possible to use Lumiprobe antibody labeling kit to achieve labeling degree of 2-3 dye molecules per Ab.
Updated 1 year ago
How to perform the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit?
In this article is in details described, how to take the measurements with Qubit™ fluorometer using QuDye Protein Quantification kit.
Updated 1 year ago
How to attach DBCO (ADIBO) group to an antibody?
Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.
Updated 1 year ago
Do you have any data to show how good it is for Pico488 to bind the dsDNA than the ssDNA?
Specificity of Pico488 for binding dsDNA versus ssDNA.
Updated 1 year ago
What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?
The solubility of Cyanine7 derivatives in different organic solvents.
Updated 1 year ago
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Non-sulfonated versus sulfonated dyes for proteins labeling with our dyes.
Updated 2 years ago
What does "minimal" mean in Cyanine NHS ester minimal dye products and can Cyanine2, Cyanine3 and Cyanine5 be mixed all together?
"Minimal" meaning in Cyanine NHS ester minimal dye products for DIGE applications
Updated 2 years ago
How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?
Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit
Updated 2 years ago
Is it possible to use Pico488 quantification solution or kit with Qubit™ reader?
Short description how to replace the Qubit™ dsDNA HS Assay Kit by the Pico488 kit/reagent.
Updated 2 years ago
What is the structure of the dye in Pico488 DNA quantification solution?
The chemical structure for dye used in Pico488 solution.
Updated 2 years ago
What is the best practice of Electrophoresis gel staining with dsGreen?
In this article we have compared different methods of using dsGreen gel staining dye to indicate difference between EtBr and DsGreen.
Updated 2 years ago
How to use passive PCR reference dye?
This is a short description of the best practice to use ROX ref. dye in PCR experiments.
Updated 2 years ago
How do I calculate the optimal amounts and procedure to label protein/antibody?
This article as about fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.
Updated 2 years ago
Is it possible to label Tyrosine containing peptides?
Labeling of the Tyr containing peptides is possible according to literature.
Updated 2 years ago
Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?
Using distilled water for maleimide labeling
Updated 2 years ago
What is the proper color of Cyanine5 SE - succinimidyl ester?
This article is to explain possible appearance and colours range you may observe for Cyanine 5 NHS ester powder/thin films.
Updated 2 years ago
Is NIR dye visible in the day light?
Is there any visible colour change when excited?
Updated 2 years ago
How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?
Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.
Updated 2 years ago
What is hydrolysis rates for NHS Ester functionalized dyes?
NHS ester tends to hydrolyze over the time in water.
Updated 2 years ago