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PCR/qPCR

Polymerase chain reaction related questions

Alexander FomenkovMarina Goryunova

4 articles in this category / Updated 8 months ago

Written by 3 authors

Quantitative real-time PCR with dsGreen?
Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes

Updated 8 months ago

How to use passive PCR reference dye?
How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments

Updated 10 months ago

Oligonucleotides delivered! What's next?
Oligonucleotides delivered! What's next?

Here, we describe how to prepare stock solutions of Lumiprobe's oligonucleotides

Updated 10 months ago

Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?
Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers

Updated 10 months ago