PCR/qPCR

Polymerase chain reaction related questions

4 articles in this category / Updated 6 days ago

Written by Alexander Fomenkov and Marina Goryunova

Quantification in 96-well Fluorescent Microplate reader

The measurement of DNA concentration in microplates has a significant advantage in perfomance of analysis. Here are some recommendations about dsDNA quantification in 96-well plates using the Pico488 dye and fluorescence microplate reader.

Updated 6 days ago

Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.

Updated 1 month ago

How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments.

Updated 3 months ago

Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers

Updated 2 years ago