PCR/qPCR

Polymerase chain reaction related questions

Alexander FomenkovMarina GoryunovaSvetlana Uglanova

5 articles in this category / Updated 5 days ago

Written by 4 authors

Recommendations for primers and probes design
Recommendations for primers and probes design

User guidelines for selecting primers and probes for polymerase chain reaction (PCR)

Updated 5 days ago

Oligonucleotides delivered! What's next?
Oligonucleotides delivered! What's next?

Here, we describe how to prepare stock solutions of Lumiprobe's oligonucleotides

Updated 1 month ago

Quantitative real-time PCR with dsGreen?
Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes

Updated 7 months ago

Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?
Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers

Updated 8 months ago

How to use passive PCR reference dye?
How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments

Updated 8 months ago