PCR/qPCR

Polymerase chain reaction related questions

Alexander FomenkovMarina Goryunova

4 articles in this category / Updated 11 months ago

Written by Alexander Fomenkov and Marina Goryunova

Quantitative real-time PCR with dsGreen?
Quantitative real-time PCR with dsGreen?

Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes.

Updated 1 year ago

How to use passive PCR reference dye?
How to use passive PCR reference dye?

This is a short description of the best practice to use ROX ref. dye in PCR experiments.

Updated 1 year ago

Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?
Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers

Updated 3 years ago