PCR/qPCR

Polymerase chain reaction related questions

Alexander FomenkovMarina Goryunova

2 articles in this category / Updated diff.ago.month

Written by Alexander Fomenkov and Marina Goryunova

Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?
Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers

Updated diff.ago.month

Quantification in 96-well Fluorescent Microplate reader
Quantification in 96-well Fluorescent Microplate reader

The measurement of DNA concentration in microplates has a significant advantage in perfomance of analysis. Here are some recommendations about dsDNA quantification in 96-well plates using the Pico488 dye and fluorescence microplate reader.

Updated diff.ago.month