This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.
Updated 1 month ago
Protein labeling
Protein labeling related questions
How to get a fluorescent derivative of biomolecule using dye hydrazide?
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Non-sulfonated versus sulfonated dyes for proteins labeling with Lumiprobe dyes.
Updated 3 months ago
Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?
Even when the concentration of Ab in the solution is below the recommended concentration of 1 mg/mL, it is still possible to use the Lumiprobe antibody labeling kits to achieve a labeling degree of 2-3 dye molecules per Ab.
Updated 3 months ago
Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction
Copper-free click chemistry conjugation guidelines, considerations, and reaction scheme.
Updated 3 months ago
How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?
Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit
Updated 3 months ago
How to attach DBCO (ADIBO) group to an antibody?
Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.
Updated 3 months ago
Do you have a protocol for labeling proteins with hydrazide-based dyes?
Brief protocol for protein labeling using hydrazide functionalized dyes.
Updated 3 months ago
How do I calculate the optimal amounts and procedure to label protein/antibody?
This article is about a fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.
Updated 3 months ago
Is it possible to label Tyrosine-containing peptides?
Labeling of the Tyr-containing peptides is possible according to the literature.
Updated 3 months ago