Protein labeling

Protein labeling related questions

Alexander FomenkovNikita OskolkovMarina Goryunova

9 articles in this category / Updated 1 month ago

Written by 3 authors

How to get a fluorescent derivative of biomolecule using dye hydrazide?
How to get a fluorescent derivative of biomolecule using dye hydrazide?

This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.

Updated 1 month ago

Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?

Non-sulfonated versus sulfonated dyes for proteins labeling with Lumiprobe dyes.

Updated 3 months ago

Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?
Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?

Even when the concentration of Ab in the solution is below the recommended concentration of 1 mg/mL, it is still possible to use the Lumiprobe antibody labeling kits to achieve a labeling degree of 2-3 dye molecules per Ab.

Updated 3 months ago

Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction
Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction

Copper-free click chemistry conjugation guidelines, considerations, and reaction scheme.

Updated 3 months ago

How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?
How much of dye is in each reaction Sulfo-Cyanine3 antibody labeling kit?

Amount of dye per reaction in Sulfo-Cyanine3 antibody labeling kit

Updated 3 months ago

How to attach DBCO (ADIBO) group to an antibody?
How to attach DBCO (ADIBO) group to an antibody?

Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.

Updated 3 months ago

Do you have a protocol for labeling proteins with hydrazide-based dyes?
Do you have a protocol for labeling proteins with hydrazide-based dyes?

Brief protocol for protein labeling using hydrazide functionalized dyes.

Updated 3 months ago

How do I calculate the optimal amounts and procedure to label protein/antibody?
How do I calculate the optimal amounts and procedure to label protein/antibody?

This article is about a fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.

Updated 3 months ago

Is it possible to label Tyrosine-containing peptides?
Is it possible to label Tyrosine-containing peptides?

Labeling of the Tyr-containing peptides is possible according to the literature.

Updated 3 months ago