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Protein labeling

Protein labeling related questions

Alexander FomenkovNikita OskolkovMarina Goryunova

10 articles in this category / Updated 2 months ago

Written by 3 authors

How much of dye is in each reaction sulfo-Cyanine3 antibody labeling kit?
How much of dye is in each reaction sulfo-Cyanine3 antibody labeling kit?

Amount of dye per reaction in sulfo-Cyanine3 antibody labeling kit

Updated 2 months ago

Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction
Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction

Copper-free click chemistry conjugation guidelines, considerations, and reaction scheme.

Updated 3 months ago

Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?

Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).

Updated 3 months ago

How to get a fluorescent derivative of biomolecule using dye hydrazide?
How to get a fluorescent derivative of biomolecule using dye hydrazide?

This article provides detailed protocol for labeling of hyaluronic acid and antibody through periodate oxidation using Cyanine5 hydrazide.

Updated 5 months ago

Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?

Non-sulfonated versus sulfonated dyes for proteins labeling with Lumiprobe dyes.

Updated 7 months ago

Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?
Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?

Even when the concentration of Ab in the solution is below the recommended concentration of 1 mg/mL, it is still possible to use the Lumiprobe antibody labeling kits to achieve a labeling degree of 2-3 dye molecules per Ab.

Updated 7 months ago

How to attach DBCO (ADIBO) group to an antibody?
How to attach DBCO (ADIBO) group to an antibody?

Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.

Updated 7 months ago

Do you have a protocol for labeling proteins with hydrazide-based dyes?
Do you have a protocol for labeling proteins with hydrazide-based dyes?

Brief protocol for protein labeling using hydrazide functionalized dyes.

Updated 7 months ago

How do I calculate the optimal amounts and procedure to label protein/antibody?
How do I calculate the optimal amounts and procedure to label protein/antibody?

This article is about a fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.

Updated 7 months ago

Is it possible to label Tyrosine-containing peptides?
Is it possible to label Tyrosine-containing peptides?

Labeling of the Tyr-containing peptides is possible according to the literature.

Updated 7 months ago