AF 488 TCO

Cat. # Quantity Price Lead time
118H0 1 mg $199 in stock
218H0 5 mg $649 in stock
418H0 25 mg $1789 in stock
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Trans-cyclooctene (TCO) is one of the most used cycloalkenes for bioconjugation tasks. TCO readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term aqueous stability, which is important in low-concentration applications such as protein-protein conjugations, etc.

This TCO is a AF 488-containing derivative. AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: orange powder
Molecular weight: 989.27
Molecular formula: C48H70N6O12S2
Solubility: good in water, DMSO; moderate in acetonitrile
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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