AF 488 tyramide

AF 488 Tyramides Microscopy
Cat. # Quantity Price Lead time
3805-1mg 1 mg $150 in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.

This tyramide is a conjugate of the water-soluble green fluorescent dye AF 488. AF 488 tyramide is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

AF 488 tyramide, 3805-1mg
AF 488 tyramide, 3805-1mg
AF 488 tyramide, 3805-1mg
Structure of AF 488 tyramide
Download in
ChemDraw format

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

Recommended protocol

Calculator

Customers also purchased with this product

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared fluorescent dye, an improved analog of Cy7®.

DiI, lipophilic tracer

Orange-red fluorescent lipophilic dye for membrane labeling and cell tracing.

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for сlick сhemistry.

General properties

Appearance: orange solid
Molecular weight: 856.03
Molecular formula: C41H53N5O11S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.