Cyanine3 maleimide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
11080 | 1 mg | $125 | in stock | |
21080 | 5 mg | $260 | in stock | |
41080 | 25 mg | $510 | in stock | |
51080 | 50 mg | $895 | in stock | |
61080 | 100 mg | $1490 | in stock |
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
Cy3 absorbance and emission spectra
Recommended protocol
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Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.TAMRA azide, 5-isomer
Azide derivative of TAMRA fluorescent dye for сlick chemistry.General properties
Appearance: | red powder |
Molecular weight: | 666.56 |
Molecular formula: | C36H43N4O3BF4 |
Solubility: | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 555 |
ε, L⋅mol−1⋅cm−1: | 150000 |
Emission maximum, nm: | 570 |
Fluorescence quantum yield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |
Product citations
- Fischer, J.M.; Stewart, M.; Dai, M.; Drennan, S.; Holland, S.; Quentel, A.; Sabuncu, S.; Kingston, B.R.; Dengos, I.; Xiang, L.; Bonic, K.; Goncalves, F.; Yi, X.; Ranganathan, S.; Branchaud, B.P.; Muldoon, L.L.; Barajas, R.F.; Yildirim, A. Peptide Amphiphiles Hitchhike on Endogenous Biomolecules for Enhanced Cancer Imaging and Therapy. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.21.580762
- Ernst, M.; Orabi, E.A.; Stockbridge, R.B.; Faraldo-Gómez, J.D.; Robertson, J.L. Dimerization mechanism of an inverted-topology ion channel in membranes. Proceedings of the National Academy of Sciences of the United States of America, 2024, 120(47), e2308454120. doi: 10.1073/pnas.2308454120
- Johnson, J.L.; Steele, J.H.; Lin, R.; Stepanov, V.G.; Gavriliuc, M.N.; Wang, Y. Multi-Channel smFRET study reveals a Compact conformation of EF-G on the Ribosome. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.27.577133
- Chadda, R.; Lee, T.; Mahoney-Kruszka, R.; Kelley, E. G.; Bernhardt, N.; Sandal, P.; Robertson, J. L. A Thermodynamic Analysis of CLC Transporter Dimerization in Lipid Bilayers. Proceedings of the National Academy of Sciences, 2023, 120(41), e2305100120. doi: 10.1073/pnas.2305100120
Cy® is a trademark of GE Healthcare.
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