exo-BCN CE-phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
4396-100mg | 100 mg | – | 12 days | |
4396-250mg | 250 mg | $790 | 12 days | |
4396-1g | 1 g | $2340 | 12 days | |
4396-5g | 5 g | please inquire | 12 days |
BCN-containing phosphoramidite is added at the 5’-terminus of an oligonucleotide. BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA).
Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).
General properties
Appearance: | yellowish oil |
Mass spec M+ increment: | 343.11 |
Molecular weight: | 481.57 |
Molecular formula: | C24H40N3O5P |
Solubility: | good in acetonitrile |
Quality control: | NMR 1H, NMR 31P (95 %) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |