LumiTracker® Lyso Blue
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A8201 | 50 uL, 1 mM/DMSO | $22 | in stock |
LumiTracker® Lyso Blue is a blue-emitting dye that selectively accumulates in cellular organelles with acidic contents. It is widely used to stain live lysosomes that maintain a low internal pH by an ATP-dependent proton pump providing optimal conditions for the activity of digestive enzymes.
LumiTracker® Lyso Blue contains several amino groups that act as a weak base. At neutral pH, they are not completely protonated, and the dye molecule remains relatively neutral, thus allowing better cell membrane permeability. Getting into lysosomes with an acidic pH, the dye is likely to become protonated and thus remains in the organelle with a low pH.
LumiTracker® Lyso Blue exhibits an absorption maximum at 375 nm and an emission maximum at 425 nm. We also offer LumiTracker® Lyso Green and LumiTracker® Lyso Red for lysosome imaging in the green and red channels.
LumiTracker® Lyso Blue is supplied as a 1 mM solution in DMSO.
Usage
Working concentration: 75–500 nM.*
Staining: 30 minutes at 37 °C in 1× PBS (just before visualization).
*Depending on the cell line, working concentration or incubating time may be changed
Product in action
Live MDCK renal epithelial cells stained with LumiTracker Lyso Blue (x8201), PKH26 (x3201), and LUCS 13 (x4010) dyes
Live MDCK renal epithelial cells stained with LumiTracker Lyso Blue (x8201) and LUCS 13 (x4010) dyes
Absorption and emission spectra of LumiTracker® Lyso Blue
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Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.General properties
| Appearance: | yellow liquid |
| Molecular weight: | 524.41 |
| CAS number: | 215247-93-1 |
| Molecular formula: | C24H38Cl4N4 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 375 |
| Emission maximum, nm: | 425 |
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