MitoCLox, mitochondrial lipid peroxidation probe

Mitochondria Cell viability and apoptosis Microscopy Flow cytometry
Cat. # Quantity Price Lead time
3549-50ug 50 ug –   in stock
3549-500ug 500 ug $260 in stock
3549-1mg 1 mg $495 in stock
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During the ferroptosis and mitochondrial stage of apoptosis, a mitochondria-specific phospholipid, cardiolipin (CL), undergoes peroxidation. MitoCLox is a mitochondria-targeted fluorescence probe that allows monitoring of this process in vivo.

MitoCLox consists of the BDP 581/591 fluorophore carrying a diene-containing moiety (C11) and linked with a triphenylphosphonium (TPP) residue via a long flexible linker with two amide bonds. MitoCLox is similar to MitoPerOx but has a longer linker and contains two (vs. one in MitoPerOx) peptide bonds. The flexible linker of MitoCLox mimics the SS-20 peptide (Phe-D-Arg-Phe-Lys-NH2), making the indicator specific for cardiolipin. The linker also increases the cellular permeability of MitoCLox due to additional positive charges.

The oxidation of the diene in MitoCLox results in a substantial increase in the fluorescence emission at 520 nm and a decrease in the initial fluorescence at 590 nm of the BDP 581/591 fluorophore. Thus, the oxidation of MitoCLox could be measured either as a decrease of absorbance at 588 nm or as an increase of fluorescence emission in the ratiometric mode at 520/590 nm [1].

MitoCLox is accumulated in the mitochondria of living cells. Maximal accumulation of MitoCLox in the cells is reached in 45-60 min. After removing MitoCLox from the medium, the fluorescence of the cells slowly decreased and reached 50% of the maximum in approximately 1 h. The recommended working concentration of MitoCLox is 100-200 nM [2].


[1] Lyamzaev K.G. et al. MitoCLox: A Novel Mitochondria-Targeted Fluorescent Probe for Tracing Lipid Peroxidation. Oxid. Med. Cell Longev. 2019:9710208.

[2] Lyamzaev K.G. et al. Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo. Oxid. Med. Cell Longev. 2020:3631272.
Monitoring mitochondrial cardiolipin peroxidation in vivo using the MitoCLox probe. HeLa cells were incubated for 1 h with 100 μM tert-butyl hydroperoxide, followed by 30 min with 200 nM MitoCLox. Cells were analyzed using a Leica TCS SP8 SMD confocal microscope with a 63x HCPL Apo 63x/1.2W CS2 objective using the green (excitation at 448 nm and emission at 500-560 nm) and red channels (excitation at 559 nm and emission at 580-630 nm). For the obtained images, the signal ratio in the green/red channels was calculated for each cell. Scale 10 μm.
MitoCLox, mitochondrial lipid peroxidation probe, 3549-1mg
MitoCLox, mitochondrial lipid peroxidation probe, 3549-1mg
MitoCLox, mitochondrial lipid peroxidation probe, 3549-1mg
Structure of MitoCLox
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Absorption and emission spectra of MitoCLox in oxidized form

Absorption and emission spectra of MitoCLox in oxidized form

Absorption and emission spectra of MitoCLox

Absorption and emission spectra of MitoCLox

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General properties

Appearance: black powder
Molecular weight: 901.69
Molecular formula: C50H53BBrF2N4O2P
Solubility: good in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 585
ε, L⋅mol−1⋅cm−1: 138500
Emission maximum, nm: 595
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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