Phosphate CPG 500 v2

Cat. # Quantity Price Lead time
17860 100 mg $40 in stock
47860 1 g $170 in stock
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Phosphate CPG 500 v2 allows direct preparation of oligonucleotides with a 3’-phosphate group.

The sulfone linker is one of the linkers employed for the preparation of oligonucleotides, it releases the oligonucleotide 3’-phosphate by β-elimination upon ammonolysis.

Available in different pore sizes, Phosphate CPG 500 v2 with pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 30 bases.

For synthesis longer oligonucleotides 1000 Å CPG can be used.

Usage

Coupling: identical to normal protected nucleoside support.

Deprotection: identical to protected nucleobases.

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General properties

Appearance: slightly yellow beads
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50-80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: identical to protected nucleobases
Deprotection conditions: identical to protected nucleobases
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