RH 237, potentiometric probe

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3734-5mg 5 mg $139 in stock
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RH 237, also called N-(4-sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, is a fast-response potential-sensitive probe. RH 237 is primarily used for imaging membrane potential, synaptic activity, and ion channel activity of neurons. However, the dye is also suitable for imaging the functional activity of mitochondria and heart cells.

Excitation/emission maxima of RH 237 in ethanol are 532/777 nm, respectively. In cell membranes, the spectra of the dye are typically blue-shifted by approximately 20 nm for excitation and 80 nm for emission peaks.

Use 1-5 μM working concentration as a starting point. The exact dye concentration should be defined experimentally.

Absorption and emission spectra of RH 237

Absorption and emission spectra of RH 237

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General properties

Appearance: dark violet powder
Molecular weight: 496.72
CAS number: 83668-91-1
Molecular formula: C29H40N2O3S
IUPAC name: N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt
Solubility: water, methanol, DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 532
Emission maximum, nm: 777
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