sulfo-Cyanine7.5 NHS ester

NHS esters Protein labeling Near infrared (NIR) imaging sulfo-Cyanine7.5
Cat. # Quantity Price Lead time
16320 1 mg $125 in stock
26320 5 mg $325 in stock
46320 25 mg $850 in stock
56320 50 mg $1490 in stock
66320 100 mg $1990 in stock
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sulfo-Cyanine7.5 is a near-infrared water-soluble and hydrophilic dye for the NIR imaging applications. The structure and spectra of the dye resemble indocyanine green (ICG) which has been approved for use in humans for years. However, unlike ICG, sulfo-Cyanine7.5 contains a trimethylene bridge that increases its quantum yield compared to ICG and has a linker arm for its attachment to proteins, peptides, and other molecules. This derivative is an NHS ester for the modification of amine groups.

sulfo-Cyanine7.5 NHS ester
sulfo-Cyanine7.5 NHS ester
sulfo-Cyanine7.5 NHS ester
Structure of sulfo-Cyanine7.5 NHS ester
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Absorption and emission spectra of sulfo-Cyanine7.5

Absorption and emission spectra of sulfo-Cyanine7.5

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General properties

Appearance: dark green solid
Mass spec M+ increment: 950.2
Molecular weight: 1180.47
Molecular formula: C49H48N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 778
ε, L⋅mol−1⋅cm−1: 222000
Emission maximum, nm: 797
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.09

Product citations

  1. Colville, M.J.; Huang, L.-T.; Schmidt, S.; Chen, K.; Vishwanath, K.; Su, J.; Williams, R.M.; Bonassar, L.J.; Reesink, H.L.; Paszek, M.J. Recombinant manufacturing of multispecies biolubricants. bioRxiv, 2024, preprint. doi: 10.1101/2024.05.05.592580
  2. Khadria, A.; Paavola, C.D.; Maslov, K.; Brown-Augsburger, P.L.; Grealish, P.F.; Lozano, E.; Blankenship, R.L.; Cao, R.; Shi, J.; Beals, J.M.; Oladipupo, S.S.; Wang, L.V. Photoacoustic imaging of the dynamics of a dye-labeled IgG4 monoclonal antibody in subcutaneous tissue reveals a transient decrease in murine blood oxygenation under anesthesia. Journal of Biomedical Optics, 2023, 28(11), 116002. doi: 10.1117/1.jbo.28.11.116002
  3. Shevchuk, A. I.; Svinko, V. O.; Smirnov, A. N.; Solovyeva, E. V. SERS Study of Cyanine Dyes: Optimization of Metal Core and Molecular Label Choice for Plasmonic Nanotags. Dyes and Pigments, 2023, 216, 111329. doi: 10.1016/j.dyepig.2023.111329.
  4. Musicò, A.; Zenatelli, R.; Romano, M.; Zendrini, A.; Alacqua, S.; Tassoni, S.; Paolini, L.; Urbinati, C.; Rusnati, M.; Bergese, P.; Pomarico, G.; Radeghieri, A. Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable” † † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3na00280b. Nanoscale Advances, 2023, 5(18), 4703-4717. doi: 10.1039/d3na00280b
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