sulfo-Cyanine5 tyramide

Cat. # Quantity Price Lead time
3796-1mg 1 mg $110 in stock
Found better price? Let us know and we will propose the way forward!

Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.

This tyramide is a conjugate of the water-soluble far-red fluorescent dye sulfo-Cyanine5. sulfo-Cyanine5 tyramide (also known as Cy5® and Cyanine5 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

Absorption and emission spectra of sulfo-Cyanine5

Absorption and emission spectra of sulfo-Cyanine5

Customers also purchased with this product

LumiMount DAPI Fluorescence Mounting Medium

An aqueous anti-fade mounting medium with DAPI for coverslipping and imaging fluorescently labeled cell or tissue samples.

BDP® FL ceramide

Fluorescent derivative of sphingosine with emission in the green spectrum region for visualization of the Golgi apparatus in cells.

DAPI, blue fluorescent nucleic acid stain

DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent DNA dye used for chromosome staining and as a nuclear counterstain in fluorescence microscopy and flow cytometry.

General properties

Appearance: dark blue solid
Molecular weight: 800.05
Molecular formula: C40H46KN3O8S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.