TAMRA-2,4-dinitroaniline (TMR-DN)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2641-1mg | 1 mg | $190 | in stock | |
| 2641-5mg | 5 mg |
$695
|
in stock | |
| 2641-10mg | 10 mg |
$990
|
in stock | |
| 2641-100mg | 100 mg | please inquire | in stock |
TMR DN is a probe, the 5-carboxy tetramethylrhodamine (TMR) linked with a quencher moiety, dinitroaniline (DN), enabling contact quenching, non-toxic for cells, and capable cell membrane penetration.
The core of the molecule is represented by an aromatic fragment bearing a negatively charged carboxylic group, it possesses bright fluorescence properties, and at the same time, it reduces nonspecific binding to genomic DNA or RNA.
RNA-based sensors and TMR DN are used for intracellular visualization of mRNA and rRNA in live prokaryotic or eukaryotic cells. Tandem repeat aptamers (e.g., sulforhodamine-binding RNA aptamer (SRB-2)) may provide an advantage for the visualization of less stable RNA and those of low content quantities in cells. The advantage of the SRB-2/TMR DN system is its high brightness, comparable to GFP (green fluorescent protein), showing a smaller complex size. SRB-2 is also orthogonal to Spinach/Broccoli aptamers, and no crossreactivity between aptamers and ligands (TMR DN and DFHBI ((3,5-difluoro-4-hydroxybenzylidene)) occurs, thus TMR DN and SRB-2 can be used along with the other probe/aptamer pairs for visualization and dynamic imaging in parallel for several RNA [1]. The excitation and emission maxima of the complex SRB-2/TMR DN are 561 and 587 nm, respectively, lying in the orange region of the spectrum where the cellular auto-fluorescence is low [2].
[1] Rigumula Wu et al. Ratiometric Fluorogenic RNA-Based Sensors for Imaging Live-Cell Dynamics of Small Molecules. ACS Applied Bio Materials. 2020. 3(5). 2633-2642.
[2] Murat Sunbul & Andres Jäschke. SRB-2: a promiscuous rainbow aptamer for live-cell RNA imaging. Nucleic acids research. 2018. 46(18).
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| Appearance: | red crystals |
| Molecular weight: | 726.73 |
| Molecular formula: | C37H38N6O10 |
| IUPAC name: | 3',6'-bis(dimethylamino)-N-(2-(2-(2-((2,4-dinitrophenyl)amino)ethoxy)ethoxy)ethyl)-3-oxo-3',9a'-dihydro-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxamide |
| Solubility: | soluble in DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
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