TET phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| C8160 | 100 mg | – | in stock | |
| D8160 | 250 mg | $197 | in stock | |
| F8160 | 1 g |
$575
|
in stock | |
| G8160 | 5 g |
$2265
|
in stock | |
| H8160 | 10 g | please inquire | in stock |
TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.
TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).
TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).
5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.
Recommendations for using the reagent:
Condensation: 3 min.
Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C).
Absorption and emission spectra of TET
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General properties
| Appearance: | white solid foam |
| Molecular weight: | 981.72 |
| CAS number: | 877049-90-6 |
| Molecular formula: | C46H54N3Cl4O10P |
| IUPAC name: | 2',4,7,7'-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate) |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 100000 |
| Emission maximum, nm: | 535 |
| Fluorescence quantum yield: | 0.47 |
| CF260: | 0.17 |
| CF280: | 0.09 |
Oligo synthesis details
| Diluent: | anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week). |
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