AF 488 carboxylic acid
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11890 | 1 mg | $125 | in stock | |
| 21890 | 5 mg | $325 | in stock | |
| 41890 | 25 mg |
$950
|
in stock | |
| 51890 | 50 mg |
$1625
|
in stock | |
| 61890 | 100 mg |
$2090
|
in stock |
AF 488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF 488 can be used with DAPI and is well suited to multiplex assay. AF 488 has high quantum yield and stable fluorescence within the pH range from 4 to 10.
AF 488 carboxylic acid is a non-reactive AF 488 form that can be used as a reference standard in experiments where AF 488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF 488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.
Absorption and emission spectra of AF 488
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| Appearance: | orange crystals |
| Molecular weight: | 648.75 |
| Molecular formula: | C21H11K3N2O11S2 |
| IUPAC name: | 4-(6-amino-3-iminio-4,5-disulfonato-3H-xanthen-9-yl)isophthalate |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
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