AF 568 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A5820 | 1 mg | $140 | in stock | |
| B5820 | 5 mg |
$450
|
in stock | |
| C5820 | 10 mg |
$585
|
in stock | |
| D5820 | 25 mg |
$1460
|
in stock | |
| E5820 | 50 mg |
$1890
|
in stock | |
| F5820 | 100 mg |
$2990
|
in stock |
AF 568 is a fluorescent dye with excitation peak at 572 nm and emission peak at 598 nm. AF 568 azide is a photochemically stable, specific, and highly efficient tool for labeling biomolecules. It is water soluble and insensitive to pH changes between pH 4 and pH 10. The reaction conditions do not require high temperature or pressure.
Labeling with AF 568 azide via click chemistry is a powerful technique for the production of bioconjugates. AF 568 azide is an excellent tool for imaging purposes, including fluorescent microscopy and flow cytometry, where label brightness and photostability are essential.
Absorption and emission spectra of AF 568
Recommended protocol
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Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 776.2 |
| Molecular weight: | 853.02 |
| Molecular formula: | C36H34N6K2O10S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 572 |
| ε, L⋅mol−1⋅cm−1: | 94238 |
| Emission maximum, nm: | 598 |
| Fluorescence quantum yield: | 0.912 |
| CF260: | 0.4 |
| CF280: | 0.32 |
Product citations
- Huber, J.; Tanasie, N.-L.; Zernia, S.; Stigler, J. Single-molecule imaging reveals a direct role of CTCF’s zinc fingers in SA interaction and cluster-dependent RNA recruitment. Nucleic Acids Research, 2024, 52(11), 6490-6506. doi: 10.1093/nar/gkae391
- Rapp, T.L.; DeForest, C.A. Tricolor visible wavelength-selective photodegradable hydrogel biomaterials. Nature Communications, 2023, 14, 5250. doi: 10.1038/s41467-023-40805-w
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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