Cyanine5.5 phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2680-100mg | 100 mg | – | in stock | |
| 2680-250mg | 250 mg | $290 | in stock | |
| 2680-1g | 1 g |
$950
|
in stock | |
| 2680-10g | 10 g | please inquire | 5 days |
Cyanine5.5 is a fluorophore with an emission in the far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in an oligonucleotide synthesizer.
The structure of the phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintain coupling performance over a longer storage time in oligonucleotide synthesizers, compared to phosphoramidites derived from primary alcohols.
Absorption and emission spectra of Cyanine5.5
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FAM phosphoramidite, 5-isomer
Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 916.61 |
| Molecular formula: | C55H71N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 694 |
| ε, L⋅mol−1⋅cm−1: | 198000 |
| Emission maximum, nm: | 710 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.07 |
| CF280: | 0.03 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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