Cyanine5.5 phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2680-100mg | 100 mg | – | in stock | |
2680-250mg | 250 mg | $290 | in stock | |
2680-1g | 1 g | $950 | in stock | |
2680-10g | 10 g | please inquire | 5 days |
Cyanine5.5 is a fluorophore with the emission in far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
Absorption and emission spectra of Cyanine5.5
Customers also purchased with this product
Universal CPG type II, 1000A
This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.dsGreen for Real-Time PCR, 100×
Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.FAM phosphoramidite, 6-isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.General properties
Appearance: | dark colored solid |
Molecular weight: | 916.61 |
Molecular formula: | C55H71N5ClO3P |
Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 694 |
ε, L⋅mol−1⋅cm−1: | 198000 |
Emission maximum, nm: | 710 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |