BO-TAP-1, cyan fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A3910 50 uL, 5 mM/DMSO $85 in stock
23910 200 uL, 5 mM/DMSO $320 in stock
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BO-TAP-1 (also known as BO-PRO®-1) is a cyan fluorescent carbocyanine monomeric dye. BO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make BO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to detect and count cells (megakaryocyte, erythroblasts, sperms, microorganisms, etc.) and bacteria, perform nucleic acid detection, sequencing, and amplification and as a temperature sensor.

Excitation and emission spectra of BO-TAP-1 (DNA–dye complex)

Excitation and emission spectra of BO-TAP-1 (DNA–dye complex)

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General properties

Appearance: yellow solution
Molecular weight: 595.33
CAS number: 157199-57-0
Molecular formula: C20H27I2N3S
IUPAC name: trimethyl-[3-[4-[(Z)-(3-methyl-1,3-benzothiazol-2-ylidene)methyl]pyridin-1-ium-1-yl]propyl]azanium;diiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 454 (complex)
Emission maximum, nm: 487 (complex)
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