PO-TAP-1, blue fluorescent nucleic acid stain
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A3810 | 50 uL, 5 mM/DMSO | $85 | in stock | |
23810 | 200 uL, 5 mM/DMSO | $320 | in stock |
PO-TAP-1 (Oxazole Blue Monomer, also known as PO-PRO®-1) is a blue fluorescent carbocyanine monomeric dye. PO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make PO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
The dye is used to detect and count cells and microorganisms, perform nucleic acid sequencing, amplification, and hybridization, and as a temperature sensor.
Excitation and emission spectra of PO-TAP-1 (DNA–dye complex)
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Concentrated reagent for high sensitive (HS) double-stranded (dsDNA) quantification to be used in any fluorescence reader.General properties
Appearance: | red solution |
Molecular weight: | 579.26 |
CAS number: | 157199-56-9 |
Molecular formula: | C20H27I2N3O |
IUPAC name: | trimethyl-[3-[4-[(E)-(3-methyl-1,3-benzoxazol-2-ylidene)methyl]pyridin-1-ium-1-yl]propyl]azanium;diiodide |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 423 (complex) |
Emission maximum, nm: | 460 (complex) |