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CDr20 (Cell Designation red 20) is a high-performance fluorogenic chemical probe for labeling microglia in both cell cultures and live brains. CDr20 is a substrate of microglia-specific UDP-glucuronosyltransferase Ugt1a7c. The glucuronidation of CDr20 by Ugt1a7c produces bright red fluorescence in microglial cells coinciding with the expression of markers P2ry12, Csf1r, Cx3cr1, and Iba-1 [1].
CDr20 could be a valuable tool for identifying and visualizing microglia in neural disorder studies both in vitro and in vivo and for CDr20-based fluorescent-activated microglial cell sorting (FACS).
[1] Kim B. et al. Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate. Angew. Chem. Int. Ed. Engl. 2019. 58(24). 7972-7976.
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