dsGreen® for Real-Time PCR, 100×

Cat. # Quantity Price Lead time
A1010 50 uL –   in stock
11010 100 uL $39 in stock
41010 1 mL $235 in stock
71010 5 mL $510 in stock
91010 50 mL $2670 in stock
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dsGreen® is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain

Excitation and emission spectra of dsDNA complex with dsGreen

Excitation and emission spectra of dsDNA complex with dsGreen

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General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
  2. Mazunina, E.P.; Gushchin, V.A.; Kleymenov, D.A.; Siniavin, A.E.; Burtseva, E.I.; Shmarov, M.M.; Mukasheva, E.A.; Bykonia, E.N.; Kozlova, S.R.; Evgrafova, E.A.; Zolotar, A.N.; Shidlovskaya, E.V.; Kirillova, E.S.; Krepkaia, A.S.; Usachev, E.V.; Kuznetsova, N.A.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Trivalent mRNA vaccine-candidate against seasonal flu with cross-specific humoral immune response. Frontiers in Immunology, 2024, 15, 1381508. doi: 10.3389/fimmu.2024.1381508
  3. Koryukov, M.A.; Oscorbin, I.P.; Novikova, L.M.; Gordukova, M.A.; Turina, I.E.; Galeeva, E.V.; Kudlay, D.A.; Filipenko, M.L. A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses. International Journal of Molecular Sciences, 2024, 25(13), 7215. doi: 10.3390/ijms25137215
  4. Shkodenko, L.A.; Mohamed, A.-A.; Ateiah, M.; Rubel, M.S.; Koshel, E.I. A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents: Haemophilus influenzae , Neisseria meningitidis , and Streptococcus pneumoniae. International Journal of Molecular Sciences, 2024, 25(15), 8282. doi: 10.3390/ijms25158282
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