Cyanine7 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15020 | 1 mg | $125 | in stock | |
| 25020 | 5 mg | $260 | in stock | |
| 45020 | 25 mg |
$510
|
in stock | |
| 55020 | 50 mg |
$895
|
in stock | |
| 65020 | 100 mg |
$1490
|
in stock |
Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.
NIR fluorophores can be used to take advantage of near infrared window of biological tissues - increased transparency of tissues in this spectral region allows to carry out in vivo imaging.
This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.
The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.
This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.
Cy7 absorbance and emission spectra
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Activated ester for modification of proteins/antibodies/peptides followed by introduction into copper-free click chemistry reaction.
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Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.General properties
| Appearance: | dark green solid |
| Molecular weight: | 733.64 |
| CAS number: | 2408482-09-5 |
| Molecular formula: | C41H48N3BF4O4 |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 750 |
| ε, L⋅mol−1⋅cm−1: | 199000 |
| Emission maximum, nm: | 773 |
| Fluorescence quantum yield: | 0.3 |
| CF260: | 0.022 |
| CF280: | 0.029 |
Product citations
- Seo, H.S.; Han, J.-H.; Lim, J.; Bae, G.-H.; Byun, M.J.; Wang, C.-P.J.; Han, J.; Park, J.; Park, H.H.; Shin, M.; Park, T.-E.; Kim, T.-H.; Kim, S.-N.; Park, W.; Park, C.G. Enhanced Post-Surgical Cancer Treatment Using Methacrylated Glycol Chitosan Hydrogel for Sustained DNA/Doxorubicin Delivery and Immunotherapy. Biomaterials Research, 2024, 28, 0008. doi: 10.34133/bmr.0008
- Huang, Y.; Wang, J.; Mancino, V.; Pham, J.; O’Grady, C.; Li, H.; Jiang, K.; Chin, D.; Poon, C.; Ho, P.-Y.; Gyarmati, G.; Peti-Peterdi, J.; Hallows, K.R.; Chung, E.J. Oral delivery of nanomedicine for genetic kidney disease. PNAS Nexus, 2024, 3(5), pgae187. doi: 10.1093/pnasnexus/pgae187
- Son, T.; Kim, M.; Choi, M.; Nam, S.H.; Yoo, A.; Lee, H.; Han, E.H.; Hong, K.S.; Park, H.S. Advancing fluorescence imaging: enhanced control of cyanine dye-doped silica nanoparticles. Journal of Nanobiotechnology, 2024, 22, 347. doi: 10.1186/s12951-024-02638-7
- Colli, C.; Masi, I.; Jacchetti, E.; Santoni, S.; Sponchioni, M.; Colosimo, B.M.; Rosanò, L.; Raimondi, M.T.; Mauri, E.; Moscatelli, D. Zwitterionic nanoparticles for thermally activated drug delivery in hyperthermia cancer treatment. Nanoscale, 2024, 16(26), 12635-12649. doi: 10.1039/d4nr00723a
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