Cyanine7.5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16030 | 100 uL, 10 mM/DMSO | – | in stock | |
| 36030 | 500 uL, 10 mM/DMSO | $199 | in stock | |
| 46030 | 1 mL, 10 mM/DMSO |
$495
|
in stock | |
| A6030 | 1 mg | – | in stock | |
| B6030 | 5 mg | $199 | in stock | |
| C6030 | 10 mg | $297 | in stock | |
| D6030 | 25 mg |
$495
|
in stock | |
| E6030 | 50 mg |
$895
|
in stock | |
| F6030 | 100 mg |
$1490
|
in stock |
Cyanine7.5 is a NIR dye with long-wave infrared fluorescence. This derivative is azide for click chemistry.
Can be used for the construction of various labeled biomolecules containing Cyanine7.5, near-infrared fluorescent dye, and an improved analog of Cy7.5®. These conjugates can take advantage of NIR tissue transparency when used for in vivo imaging. This fluorophore is also useful for other fluorescent applications, especially requiring low fluorescent background.
Azide is available as a DMSO solution, ready for general click chemistry labeling protocol, or in solid form for custom labeling applications.
Structure features a rigid bridged polymethine chain to increase quantum yield by 20%, allowing for a brighter signal.
Cyanine7.5 absorbance and emission spectra
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AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.General properties
| Appearance: | green powder / solution |
| Molecular weight: | 767.44 |
| CAS number: | 1628790-36-2; 1628897-78-8 (without anion) |
| Molecular formula: | C48H55ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 788 |
| ε, L⋅mol−1⋅cm−1: | 223000 |
| Emission maximum, nm: | 808 |
| Fluorescence quantum yield: | 0.10 |
Product citations
- Hellyer, S.D.; Aggarwal, S.; Chen, A.N.; Leach, K.; Lapinsky, D.J.; Gregory, K.J. Development of Clickable Photoaffinity Ligands for Metabotropic Glutamate Receptor 2 Based on Two Positive Allosteric Modulator Chemotypes. ACS Chemical Neuroscience, 2020, 11(11), 1597–1609. doi: 10.1021/acschemneuro.0c00009
- Retout, M.; Brunetti, E.; Valkenier, H.; Bruylants, G. Limits of thiol chemistry revealed by quantitative analysis of mixed layers of thiolated-PEG ligands grafted onto gold nanoparticles. Journal of Colloid and Interface Science, 2019, 557, 807–815. doi: 10.1016/j.jcis.2019.09.047
- Pearce, H.A.; Qian, H.; Connell, T.U.; Huang, D.; Gottstein, C.; Donnelly, P.S.; Peter, K.; Gregorevic, P.; Hagemeyer, C.E. Site-specific glycation and chemo-enzymatic antibody sortagging for the retargeting of rAAV6 to inflamed endothelium. Molecular Therapy - Methods & Clinical Development, 2019, 14, 261–269. doi: 10.1016/j.omtm.2019.07.003
- Kong, J.-N.; Zhu, Z.; Itokazu, Y.; Wang, G.; Dinkins, M.B.; Zhong, L.; Lin, H.-P.; Elsherbini, A.; Leanhart, S.; Jiang, X.; Qin, H.; Zhi, W.; Spassieva, S.D.; Bieberich, E. Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes. Journal of Lipid Research, 2018, 59(3), 488–506. doi: 10.1194/jlr.M081877
Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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