DusQ 21 CPG 500
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
3151-100mg | 100 mg | – | in stock | |
3151-1g | 1 g | $990 | in stock |
DusQ 21 is a quencher for the dyes in far red region of the spectrum. The dye is a xanthene derivative, unlike DusQ 1 or DusQ 2, that are azo dyes. It has a high extinction coefficient, and provides an outstanding quenching efficiency.
The controlled pore glass solid support allows to synthesize oligonucleotides carrying the dye moiety on 3’-terminus.
We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of DusQ 21 quencher can be used.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or potassium carbonate K2CO3 (50 mM solution in methanol).
Absorption spectrum of DusQ 21
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Appearance: | dark blue beads |
Quality control: | 1H NMR of bound reagent, coupling testing with HPLC-MS |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 656 |
ε, L⋅mol−1⋅cm−1: | 72800 |
Oligo synthesis details
Pore size, Å: | 500 |
Typical loading, umol/g: | 50−80 |