endo-BCN CE-phosphoramidite

Cycloalkynes Oligonucleotide synthesis Copper-free click chemistry Phosphoramidites

Cat. #	Quantity	Price	Lead time
3005-100mg / in 6 mL septum	100 mg	$390	in stock
3005-250mg / in 6 mL septum	250 mg	$850	in stock
3005-1g / in 6 mL septum	1 g	$2380	in stock

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Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, bicyclononine in endo-BCN CE-phosphoramidite provides not significantly different rate of cycloaddition compared to its exo-conformer.

BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.

Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).

Structure of endo-BCN CE-Phosphoramidite

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General properties

Appearance:	yellowish oil
Mass spec M+ increment:	343.11
Molecular weight:	481.57
CAS number:	1352811-59-6
Molecular formula:	C₂₄H₄₀F₁N₃O₅P
Solubility:	good in acetonitrile
Quality control:	NMR ¹H and ³¹P (95 %)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Oligo synthesis details

Diluent:	Anhydrous Acetonitrile
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.