FAM-11-dUTP, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2077-10nmol | 10 nmol | – | in stock | |
| 2077-50nmol | 50 nmol | $190 | in stock | |
| 2077-250nmol | 250 nmol | $325 | in stock | |
| 2077-500nmol | 500 nmol |
$500
|
in stock | |
| 2077-1umol | 1 umol |
$910
|
in stock |
6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.
FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.
In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.
6-FAM-11-dUTP can be used to produce a labeled product during Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.
Absorption and emission spectra of FAM
Customers also purchased with this product
sulfo-Cyanine3 azide
Water-soluble sulfo-Cyanine3 dye azide for click chemistry labeling of sensitive molecules and intact biological objects.Cyanine5 NHS ester minimal dye
Cy5 NHS ester minimal dye for protein labeling, an analog of Cy5 minimal dye.dsGreen® for Real-Time PCR, 100×
Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.General properties
| Appearance: | yellow to orange solid |
| Molecular weight: | 1013.49 |
| Molecular formula: | C39H39N4Li3O21P3 |
| IUPAC name: | ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
Your cart is empty
$ 
