FAM alkyne, 6-isomer

FAM (Fluorescein) Alkynes Click chemistry Fluorescence polarization
Cat. # Quantity Price Lead time
A51B0 1 mg –   in stock
B51B0 5 mg $100 in stock
C51B0 10 mg $150 in stock
D51B0 25 mg $295 in stock
E51B0 50 mg $450 in stock
F51B0 100 mg $780 in stock
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Alkyne derivative of FAM (fluorescein) fluorophore for click chemistry labeling of biomolecules. Pure (97+%) single 6-isomer. Fluorescein is a bright fluorophore which is compatible with a number of different fluorescent instruments.

FAM alkyne, 6-isomer, B51B0
FAM alkyne, 6-isomer, B51B0
FAM alkyne, 6-isomer, B51B0
Structure of FAM alkyne, 6-isomer
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Absorbance and emission spectra of FAM

Absorbance and emission spectra of FAM

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Copper(II)-TBTA complex

A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.

Alkyne hydrazide

Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.

General properties

Appearance: yellow solid
Molecular weight: 413.38
CAS number: 478801-49-9
Molecular formula: C24H15O6N
Solubility: good in aqueous buffers (pH > 8), alcohols, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Hostert, J.D.; Sepesy, M. R.; Duval, C. E.;  Renner, J.N. Clickable Polymer Scaffolds Enable Ce Recovery with Peptide Ligands. Soft Matter, 2023, 19(15), 2823–2831. doi: 10.1039/D2SM01664H
  2. Klein, K.; Loza, K.; Heggen, M.; Epple, M. An Efficient Method for Covalent Surface Functionalization of Ultrasmall Metallic Nanoparticles by Surface Azidation Followed by Copper-Catalyzed Azide-Alkyne Cycloaddition (Click Chemistry). ChemNanoMat, 2021, 7(12), 1330–1339. doi: 10.1002/cnma.202100359
  3. Zisis, T.; Schwarz, J.; Balles, M.; Kretschmer, M.; Nemethova, M.; Chait, R.; Hauschild, R.; Lange, J.; Guet, C.; Sixt, M.; Zahler, S. Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal ControlACS Appl. Mater. Interfaces 2021, 13 (30), 35545–35560. https://doi.org/. ACS Appl. Mater. Interfaces, 2021, 13(30), 35545–35560. doi: 10.1021/acsami.1c09850
  4. Zisis, T.; Schwarz, J.; Balles, M.; Kretschmer, M.; Nemethova, M.; Chait, R.; Hauschild, R.; Lange, J.; Guet, C.; Sixt, M.; Zahler, S. Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control. ACS Appl. Mater. Interfaces, 2021, 13(30), 35545–35560. doi: 10.1021/acsami.1c09850
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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