FAM azide, 6-isomer

Cat. # Quantity Price Lead time
15130 100 uL, 10 mM/DMSO $69 in stock
35130 500 uL, 10 mM/DMSO $97 in stock
45130 1 mL, 10 mM/DMSO $139 in stock
A5130 1 mg $69 in stock
B5130 5 mg $97 in stock
C5130 10 mg $139 in stock
D5130 25 mg $285 in stock
E5130 50 mg $445 in stock
F5130 100 mg $765 in stock
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FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for сlick сhemistry. solid and 10 mM solution in DMSO, labeling reagent for сlick chemistry. This reagent is a pure 6-isomer.

It can replace DyLight 488.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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JOE azide, 5-isomer

JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for сlick chemistry

General properties

Appearance: yellowish crystals
Molecular weight: 458.42
CAS number: 1386385-76-7
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
  2. Gabold, B.; Adams, F.; Brameyer, S.; Jung, K.; Ried, C. L.; Merdan, T.; Merkel, O. M. Transferrin-Modified Chitosan Nanoparticles for Targeted Nose-to-Brain Delivery of Proteins. Drug Deliv. and Transl. Res., 2023, 13(3), 822–838. doi: 10.1007/s13346-022-01245-z
  3. Kafle, C. M.; Anderson, A. Y.; Prakash, A.; Swedik, S.; Bridge, E. An Adenovirus Early Region 4 Deletion Mutant Induces G2/M Arrest via ATM Activation and Reduces Expression of the Mitotic Marker Phosphorylated (Ser10) Histone 3. Virology, 2022, 565, 1–12. doi: 10.1016/j.virol.2021.09.006
  4. Babi, M.; Palermo, A.; Abitbol, T.; Fatona, A.; Jarvis, V.; Nayak, A.; Cranston, E.; Moran-Mirabal, J. Direct Imaging of the Alternating Disordered and Crystalline Structure of Cellulose Fibrils via Super-Resolution Fluorescence Microscopy. ChemRxiv, 2022, preprint. doi: 10.26434/chemrxiv-2022-0jqng
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