Cyanine5 phosphoramidite

Cat. # Quantity Price Lead time
1740-100mg 100 mg –   in stock
1740-250mg 250 mg $290 in stock
1740-1g 1 g $950 21 days
1740-10g 10 g $6750 21 days
Found better price? Let us know and we will propose the way forward!

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of the fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.

Absorption and emission spectra of Cyanine5

Absorption and emission spectra of Cyanine5

Customers also purchased with this product

SIMA phosphoramidite, 6-isomer

A phosphoramidite for the synthesis of SIMA-labeled oligonucleotides. SIMA is a more stable analog of HEX.
Add this product to your cart and
get free express delivery

TFA-amino modifier CPG 500

TFA-amino modifier CPG for oligo synthesis. 500 Å, high load controlled pore glass.

Universal CPG type II, 1000A

This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.

General properties

Appearance: dark colored solid
Molecular weight: 816.49
Molecular formula: C47H67N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.