RAPID DiO, lipophilic tracer

Neuroscience Cell membrane Microscopy Flow cytometry
Cat. # Quantity Price Lead time
3708-500ug 500 ug –   in stock
3708-5mg 5 mg $299 in stock
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RAPID DiO (also known as FAST DiO™ and Dilinoleyl DiO) is a cyanine dye with green fluorescence, an unsaturated analog of DiO (DiOC18(3)).

RAPID DiO is a lipophilic dye that labels cell membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes. RAPID DiO diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. RAPID DiO has about 50% faster migration kinetics than DiO.

RAPID DiO can be used with other tracers in dual-color studies, such as  RAPID DiI.

RAPID DiO, lipophilic tracer, 3708-5mg
RAPID DiO, lipophilic tracer, 3708-5mg
RAPID DiO, lipophilic tracer, 3708-5mg
Structure of RAPID DiO, lipophilic tracer
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Absorption and emission spectra of RAPID DiO

Absorption and emission spectra of RAPID DiO

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General properties

Appearance: orange plasticine-like
Molecular weight: 923.28
Molecular formula: C54H77F3N2O5S
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 487
ε, L⋅mol−1⋅cm−1: 166000
Emission maximum, nm: 503
Fluorescence quantum yield: 0.1
FAST DiO™ is the trademark of Invitrogen™
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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