DiO, lipophilic tracer

Neuroscience Microscopy Cell membrane Flow cytometry

Cat. #	Quantity	Price	Lead time
2936-1mg	1 mg	–	in stock
2936-5mg	5 mg	$24	in stock (MOQ 4 pcs.)
2936-50mg	50 mg	$223	in stock

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DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye. DiO’s excitation max. at 487 nm, and emission max. at 501 nm.

DiO is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiO uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.

DiO and DiI are often used together in dual-color studies. But DiO has a slower lateral diffusion rate on the membranes than that of DiI.

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Absorption and emission spectra of DiO

General properties

Appearance:	orange solid
Molecular weight:	782.27
CAS number:	28462-56-8
Molecular formula:	C₅₃H₈₅N₂O₂+
Solubility:	DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	487
ε, L⋅mol⁻¹⋅cm⁻¹:	102000
Emission maximum, nm:	501
Fluorescence quantum yield:	0.51

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.