LumiTracker® Mito Rhodamine 123

Mitochondria Cell viability and apoptosis Microscopy Flow cytometry
Cat. # Quantity Price Lead time
2958-1mg 1 mg –   in stock
2958-25mg 25 mg $105 in stock
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Rhodamine 123 (Rh123) is a cationic green-fluorescent xanthene dye used to monitor membrane polarization in mitochondria and bacteria in live cell assays.

The dye distributes according to the negative potential of the membrane. Loss of potential will result in loss of the dye and, therefore, the fluorescence intensity. Like TMRE, Rhodamine 123 can be used to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest.

Rhodamine 123 is also used as a tracer dye to determine the rate and direction of membrane transport.

LumiTracker Mito Rhodamine 123, 2958-25mg
LumiTracker Mito Rhodamine 123, 2958-25mg
LumiTracker Mito Rhodamine 123, 2958-25mg
Vital staining of mitochondria of B78 melanoma cells. Live cells were stained with nuclear dye Hoechst 33258 (blue, 1 ug/mL) for 10 min and LumiTracker Mito Rhodamine 123 (green, 2958-x) for 5 min at 37 °С, then washed to remove excess dye and visualized by confocal microscope.
Structure of Rh123
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Absorbance and emission spectra of Rh123

Absorbance and emission spectra of Rh123

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General properties

Appearance: brown crystals
Mass spec M+ increment: 345.30
Molecular weight: 380.83
CAS number: 62669-70-9
Molecular formula: C21H17ClN2O3
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 511
ε, L⋅mol−1⋅cm−1: 86000
Emission maximum, nm: 531
Fluorescence quantum yield: 0.98

Product citations

  1. Li, Z.; Zhang, L.; Zhou, L.; Li, X.; Zhao, Y.; Wang, J. Tunable Catalytic Release of Nitric Oxide via Copper-Loaded Coatings on Titanium Nanotubes for Regulating Biological Performance. Biosurface and Biotribology, 2023, 9(2), 45–57. doi: 10.1049/bsb2.12060
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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