LumiTracker® Mito TMRE

Cat. # Quantity Price Lead time
2274-1mg 1 mg –   in stock
2274-25mg 25 mg $190 in stock
Found better price? Let us know and we will propose the way forward!

TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.

In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.

TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).

Absorption and emission spectra of TMRE

Absorption and emission spectra of TMRE

Customers also purchased with this product

BDP® 650/665 lipid stain

Hydrophobic borondipyrromethene dye BDP 650/665 for staining of lipids and lipophilic compounds.

AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.

LumiTracker® Mito Orange CMTMRos

Сell-permeant, cationic, orange-fluorescent dye for staining active mitochondria in living cells in a potential-dependent manner. The stain is compatible with aldehyde fixation.

General properties

Appearance: dark colored solid
Molecular weight: 514.96
CAS number: 115532-52-0
Molecular formula: C26H27N2ClO7
IUPAC name: 3,6-bis(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthylium perchlorate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
Emission maximum, nm: 575

Product citations

  1. Mikheeva, A.M.; Bogomolov, M.A.; Gasca, V.A.; Sementsov, M.V.; Spirin, P.V.; Prassolov, V.S.; Lebedev, T.D. Improving the power of drug toxicity measurements by quantitative nuclei imaging. Cell Death Discovery, 2024, 10, 181. doi: 10.1038/s41420-024-01950-3
  2. Moiseeva, N.; Eroshenko, D.; Laletina, L.; Rybalkina, E.; Susova, O.; Karamysheva, A.; Tolmacheva, I.; Nazarov, M.; Grishko, V. The Molecular Mechanisms of Oleanane Aldehyde-β-Enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells. Biology, 2023, 12(3), 415. doi: 10.3390/biology12030415
  3. Kozlova, T.; Popov, A.; Romanov, M. V.; Savintseva, I.; Vasilyeva, D. N.; Baranchikov, A.; Ivanov, V. Ceric Phosphates and Nanocrystalline Ceria: Selective Toxicity to Melanoma Cells. Nanosystems: Physics, Chemistry, Mathematics, 2023, 14, 223–230. doi: 10.17586/2220-8054-2023-14-2-223-230
  4. Tolmacheva, I.; Beloglazova, Y.; Nazarov, M.; Gagarskikh, O.; Grishko, V. Synthesis and Anticancer Activity of A-Ring-Modified Derivatives of Dihydrobetulin. International Journal of Molecular Sciences, 2023, 24(12), 9863. doi: 10.3390/ijms24129863
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.