sulfo-Cyanine3 tyramide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3718-1mg | 1 mg | $110 | in stock |
Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble orange fluorescent dye sulfo-Cyanine3. sulfo-Cyanine3 tyramide (also known as Cy3® and Cyanine3 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.
Absorption and emission spectra of sulfo-Cyanine3
Recommended protocol
Calculator
Customers also purchased with this product
DAPI, blue fluorescent nucleic acid stain
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent DNA dye used for chromosome staining and as a nuclear counterstain in fluorescence microscopy and flow cytometry.Cyanine5 azide
Cyanine5 azide is a reagent for click chemistry labeling with Cyanine5, a popular fluorescent dye.
Add this product to your cart andget free express delivery
sulfo-Cyanine5.5 tyramide
A tyramide derivative of sulfo-Cyanine5.5, a far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | red powder |
| Molecular weight: | 774.02 |
| Molecular formula: | C38H44KN3O8S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Your cart is empty
$ 
