sulfo-Cyanine7 tyramide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3836-1mg | 1 mg | $110 | in stock |
Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble near-infrared fluorescent dye sulfo-Cyanine7. sulfo-Cyanine7 tyramide (also known as Cy7® and Cyanine7 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.
Absorption and emission spectra of sulfo-Cyanine7
Recommended protocol
Calculator
Customers also purchased with this product
Biotin-PEG4-NHS ester
PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.FAM azide, 5-isomer
FAM (fluorescein) azide for сlick chemistry labeling, pure 5-isomer.AF 488 streptavidin
AF 488 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.General properties
| Appearance: | dark blue solid |
| Molecular weight: | 866.16 |
| Molecular formula: | C45H52KN3O8S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 750 |
| ε, L⋅mol−1⋅cm−1: | 240600 |
| Emission maximum, nm: | 773 |
| Fluorescence quantum yield: | 0.24 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Your cart is empty
$ 
