AF 594 tyramide

AF 594 Microscopy Tyramides
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3804-1mg 1 mg $150 in stock
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Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.

This tyramide is a conjugate of the water-soluble red fluorescent dye AF 594. AF 594 tyramide is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

AF 594 tyramide, 3804-1mg
AF 594 tyramide, 3804-1mg
AF 594 tyramide, 3804-1mg
Structure of AF 594 tyramide
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AF 594 absorbance and emission spectra

AF 594 absorbance and emission spectra

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General properties

Appearance: dark-blue crystals
Molecular weight: 943.15
Molecular formula: C49H58N4O11S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 586
ε, L⋅mol−1⋅cm−1: 105000
Emission maximum, nm: 613
Fluorescence quantum yield: 0.77
CF260: 0.28
CF280: 0.51
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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