TAMRA NHS ester, 5-isomer

TAMRA (TMR, Tetramethylrhodamine) NHS esters Protein labeling
Cat. # Quantity Price Lead time
17120 1 mg –   in stock
27120 5 mg $210 in stock
47120 25 mg $350 in stock
57120 50 mg $595 in stock
67120 100 mg $790 in stock
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TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore has been used for quite a long time for the preparation of dual-labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).

Like many other xanthene fluorophores, TAMRA is available as two isomers (5- and 6-isomer) with nearly identical optical properties. This product is an isomerically pure 5-TAMRA.

TAMRA NHS is an amine-reactive reagent. It can be used to label proteins, peptides, and modified oligonucleotides containing amine groups.

TAMRA NHS ester, 5-isomer, 67120
TAMRA NHS ester, 5-isomer, 67120
TAMRA NHS ester, 5-isomer, 67120
Structure of 5-TAMRA NHS ester
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Absorption and emission spectra of 5-TAMRA

Absorption and emission spectra of 5-TAMRA

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TAMRA NHS ester, 6-isomer

NHS-ester of TAMRA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. Pure 6-isomer.

R6G NHS ester, 5-isomer

R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.

General properties

Appearance: dark colored solid
Molecular weight: 527.53
CAS number: 321862-17-3
Molecular formula: C29H25N3O7
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Product citations

  1. Sokolov, A. V.; Isakova-Sivak, I. N.; Mezhenskaya, D. A.; Kostevich, V. A.; Gorbunov, N. P.; Elizarova, A. Yu.; Matyushenko, V. A.; Berson, Yu. M.; Grudinina, N. A.; Kolmakov, N. N.; Zabrodskaya, Y. A.; Komlev, A. S.; Semak, I. V.; Budevich, A. I.; Rudenko, L. G.; Vasilyev, V. B. Molecular Mimicry of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein: From the Interaction of Spike-Specific Antibodies with Transferrin and Lactoferrin to the Antiviral Effects of Human Recombinant Lactoferrin. Biometals, 2023, 36(3), 437–462. doi: 10.1007/s10534-022-00458-6
  2. Chen, H.; Zhang, Y.; Ma, X.; Zhou, B.; Liu, Z. Chemically-Modified Sepharose 6B Beads for Collection of Circulating Tumor Cells. Biomolecules, 2023, 13(7), 1071. doi: 10.3390/biom13071071
  3. Bresinsky, M.; Shahraki, A.; Kolb, P.; Pockes, S.; Schihada, H. Development of Fluorescent AF64394 Analogues Enables Real-Time Binding Studies for the Orphan Class A GPCR GPR3. Journal of medicinal chemistry, 2023, 66(21), 15025-15041. doi: 10.1021/acs.jmedchem.3c01707
  4. Alexandrova, V. V.; Anisimov, M. N.; Zaitsev, A. V.; Mustyatsa, V. V.; Popov, V. V.; Ataullakhanov, F. I.; Gudimchuk, N. B. Theory of Tip Structure–Dependent Microtubule Catastrophes and Damage-Induced Microtubule Rescues. Proceedings of the National Academy of Sciences, 2022, 119(46), e2208294119. doi: 10.1073/pnas.2208294119
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