TOR-G4, G-quadruplexes fluorescent probe
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A5510 | 50 uL, 5 mM/DMSO | $149 | in stock | |
| 15510 | 500 uL, 5 mM/DMSO | $390 | in stock | |
| 4357-1g | 1 g | please inquire | in stock |
G-quadruplexes (G4s) are secondary structures that form in DNA and RNA through noncanonical hydrogen bonding between four guanine bases [1,2]. In the nucleus, DNA G4s have been associated with epigenetic regulation of gene expression through their interactions with regulatory proteins, such as transcription factors and chromatin modifiers [3,4]. RNA G4s have been linked to RNA splicing, transport, and translation regulation, as well as RNA-mediated stress responses in the cytoplasm [5-7].
TOR-G4 is a thiazole orange derivative, a newly synthesized G4 fluorescent probe [8]. It is a small-molecule alternative to immunochemistry with G4-specific antibodies. TOR-G4 allows the visualization of G4s based on changes to the fluorescence lifetime of a probe upon nucleic acid binding. The lifetime of TOR-G4 is highest in the presence of G4s and lower for other sequences. Within cells, TOR-G4 is primarily colocalized with RNA in the cytoplasm and nucleoli, making it the first lifetime-based probe validated for exploring the emerging roles of RNA G4s in cell functioning. TOR-G4 is suitable for imaging RNA G4s via FLIM [8].
[1] Trends in Chemistry 2, 123 (2020); [2] Nat Rev Mol Cell Biol 21, 459 (2020); [3] Nucleic Acids Res 49, 8419 (2021); [4] Trends Genet 35, 29 (2019); [5] Nucleic Acids Res 48, 12534 (2020); [6] Trends Biochem Sci 46, 270 (2021); [7] Nucleic Acids Res 49, 5426 (2021); [8] J Am Chem Soc 146, 1009 (2024).
Excitation and emission spectra of TOR-G4 (G4 DNA (c-MYC))
Customers also purchased with this product
DiD, lipophilic tracer
Far-red fluorescent lipophilic dye for membrane labeling and cell tracing.BDP® TMR ceramide
Fluorescent derivative of sphingosine with emission in the orange spectrum region for visualization of the Golgi apparatus in cells.Hexynoic acid NHS ester
Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.General properties
| Appearance: | black solid |
| Molecular weight: | 607.56 |
| Molecular formula: | C30H30IN3OS |
| Solubility: | good in DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 483; 567 (c-MYC complex) |
| Emission maximum, nm: | 648 (c-MYC complex) |
Your cart is empty
$ 
