Universal CPG type I, 500A
This product is discontinued, and no longer available. But we have some stock (see below)
We recommend Universal CPG type II, 500A as a substitute
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2646-1g | 1 g | please inquire | in stock (1 pcs) |
The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.
The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1) for a 50 nmol scale synthesis. The bigger scale of synthesis requires more than 4 hours to complete.
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General properties
| Appearance: | white to cream beads |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C |
| Deprotection conditions: | identical to protected nucleobases |
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