Universal CPG type I, 500A

This product is discontinued, and no longer available. But we have some stock (see below)
 We recommend Universal CPG type II, 500A as a substitute
CPG modifier solid supports Oligonucleotide synthesis
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2646-1g 1 g please inquire in stock (1 pcs)
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The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.

The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1) for a 50 nmol scale synthesis. The bigger scale of synthesis requires more than 4 hours to complete.

Universal CPG type I, 500A, 2646-1G
Universal CPG type I, 500A, 2646-1G
Universal CPG type I, 500A, 2646-1G
Structure of Universal CPG type I, 500A
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Universal CPG type II, 1000A

This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.

DusQ 21 CPG 500

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General properties

Appearance: white to cream beads
Quality control: loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C
Deprotection conditions: identical to protected nucleobases
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