DusQ 2 phosphoramidite

Cat. # Quantity Price Lead time
1473-100mg
/ Easily soluble lyophilised form in 8 mL septum
100 mg $290 in stock
1473-500mg 500 mg $990 in stock
1473-1g
/ Easily soluble lyophilised form in 30 mL septum
1 g $1690 in stock
1473-10g 10 g please inquire in stock
Found better price? Let us know and we will propose the way forward!

DusQ 2 phosphoramidite is suitable for the synthesis of oligonucleotides labeled with DusQ 2 quencher at the 5’- and 3’-ends, as well as in the middle of the sequence. This phosphoramidite has DMT protection, which allows purification of the synthesized 5’-labeled oligonucleotide on cartridges.

This phosphoramidite is commonly used for the synthesis of doubly labeled probes for quantitative PCR with DusQ 2 quencher at the 5’-end. DusQ 2 is a fluorescence quencher with maximum absorption in the range of 560–670 nm and is suitable for efficient quenching of fluorophores emitting in this range through FRET mechanism. It is also used in hybridization probes based on static and mixed quenching.

Since the quenching efficiency of DusQ 2 is minimally dependent on the spectral overlap between the fluorophore and the quencher, it is suitable for a wide range of fluorophores, including those with emission in the red and far-red ranges. The list of fluorophores for use with DusQ 2 includes, but is not limited to, Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

Absorption spectrum of DusQ 2

Absorption spectrum of DusQ 2

Customers also purchased with this product

Cyanine5.5 phosphoramidite

Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5'-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.
Add this product to your cart and
get free express delivery

Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.
Add this product to your cart and
get free express delivery

Hexynoic acid NHS ester

Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.

General properties

Appearance: dark colored solid
Molecular weight: 997.08
CAS number: 374591-98-7
Molecular formula: C54H61N8O9P
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
CF260: 0.31
CF280: 0.26

Oligo synthesis details

Diluent: 20% THF / acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.