AF 488 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| S1830 | 250 ug | – | in stock | |
| 11830 | 1 mg | $125 | in stock | |
| 21830 | 5 mg | $325 | in stock | |
| 41830 | 25 mg |
$950
|
in stock | |
| 51830 | 50 mg |
$1625
|
in stock | |
| 61830 | 100 mg |
$2090
|
in stock |
AF 488 is a fluorescent dye. AF 488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF 488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.
AF 488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.
Absorption and emission spectra of AF 488
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BDP® R6G azide
BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.Copper(II)-BTTAA complex
A catalyst for copper-catalyzed click reactions in aqueous media. A stable сopper(II) complex with BTTAA which must be converted to copper(I) using reducing agents. The complex can be used to prepare a reaction buffer of any composition.Linear Polyacrylamide for Nucleic Acid Precipitation
Nucleic acid coprecipitant. Suitable for PCR and RT-PCR reactions.General properties
| Appearance: | orange solid |
| Molecular weight: | 692.76 |
| CAS number: | 1679326-36-3 (with azidohexyl group) |
| Molecular formula: | C24H18K2N6O10S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
Product citations
- Baleva, M.V.; Piunova, U.; Chicherin, I.; Vasilev, R.; Levitskii, S.; Kamenski, P. Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells. International Journal of Molecular Sciences, 2024, 25(1), 93. doi: 10.3390/ijms25010093
- Ivanova, A.; Gruzova, O.; Ermolaeva, E.; Astakhova, O.; Itaman, S.; Enikolopov, G.; Lazutkin, A. Synthetic Thymidine Analog Labeling without Misconceptions. Cells, 2022, 11(12), 1888. doi: 10.3390/cells11121888
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