HEX azide, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
AB230 | 1 mg | $110 | in stock | |
BB230 | 5 mg | $165 | in stock | |
CB230 | 10 mg | $210 | in stock | |
DB230 | 25 mg | $285 | in stock | |
EB230 | 50 mg | $495 | in stock | |
FB230 | 100 mg | $880 | in stock |
Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.
Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.
HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Absorption and emission spectra of HEX
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Appearance: | orange solid |
Mass spec M+ increment: | 661.9 |
Molecular weight: | 665.09 |
CAS number: | 1450752-91-6 |
Molecular formula: | C24H12N4Cl6O6 |
IUPAC name: | N-(3-azidopropyl)-2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamide |
Solubility: | very soluble in DMSO, DMF |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 533 |
ε, L⋅mol−1⋅cm−1: | 87770 |
Emission maximum, nm: | 549 |
Fluorescence quantum yield: | 0.57 |
CF260: | 0.30 |
CF280: | 0.13 |