Azidobutyric acid NHS ester

NHS esters Protein labeling Crosslinking

Cat. #	Quantity	Price	Lead time
53720	50 mg	$110	in stock
63720	100 mg	$210	in stock
73720	1 g	$950	in stock
83720	5 g	please inquire	in stock

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Convert your proteins and peptides into click chemistry reactive form with this reagent.

While click chemistry involves reaction between terminal alkyne and azide, both azides and alkynes are very uncommon in nature. However, there are reagents to attach these fragments to abundant amino groups which are ubiquitous in the world of biomolecules. This azido-NHS ester is designed for the conversion of proteins, peptides, amino-DNA, and other amines into click chemistry reactive azides.

Structure of azidobutyric acid NHS ester

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Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

NHS Ester Labeling of Biomolecules Containing Primary Amino Groups

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General properties

Appearance:	colorless solid
Mass spec M+ increment:	111.0
Molecular weight:	226.19
CAS number:	943858-70-6
Molecular formula:	C₈H₁₀N₄O₄
IUPAC name:	Butanoic acid, 4-azido-, 2,5-dioxo-1-pyrrolidinyl ester
Solubility:	soluble in organic solvents (DMF, DMSO)
Quality control:	NMR ¹H (95%), HPLC
Storage conditions:	Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Product citations

Osuofa, J.; Husson, S.M. Preparation of Protein A Membranes Using Propargyl Methacrylate-Based Copolymers and Copper-Catalyzed Alkyne–Azide Click Chemistry. Polymers, 2024, 16(2), 239. doi: 10.3390/polym16020239
Skardžiūtė, K.; Kvederavičiūtė, K.; Pečiulienė, I.; Narmontė, M.; Gibas, P.; Ličytė, J.; Klimašauskas, S.; Kriukienė, E. One-pot trimodal mapping of unmethylated, hydroxymethylated, and open chromatin sites unveils distinctive 5hmC roles at dynamic chromatin loci. Cell Chemical Biology, 2024, 31(3), 607-621.e9. doi: 10.1016/j.chembiol.2023.12.003
Afting, C.; Walther, T.; Drozdowski, O. M.; Schlagheck, C.; Schwarz, U. S.; Wittbrodt, J.; Göpfrich, K. DNA Microbeads for Spatio-Temporally Controlled Morphogen Release within Organoids. Nat. Nanotechnol., 2024, 1-9. doi: 10.1038/s41565-024-01779-y
Sim, J.; Li, M.; Park, K. M.; Kim, K. Identification of Mitochondrial Proteins Associated with Cyanine 5 Using a Combination of Affinity-Based Photocrosslinking with Bio-Orthogonal Supramolecular Latching. J. Porphyrins Phthalocyanines, 2023, 27(07n10), 1148–1155. doi: 10.1142/S108842462350058X

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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.