BDP® TMR NHS ester

Cat. # Quantity Price Lead time
12420 1 mg $125 in stock
22420 5 mg $260 in stock
42420 25 mg $510 in stock
52420 50 mg $895 in stock
62420 100 mg $1490 in stock
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BDP TMR is a borondipyrromethene dye. This is an amine-reactive NHS ester for the labeling of proteins, peptides, and other molecules with amino groups.

BDP TMR is a dye for the TAMRA channel, which is, however, much brighter than TAMRA. Because of the rather long lifetime of the excited state, this fluorophore is a good choice for fluorescence anisotropy measurements, especially with the short linker offered. Fluorescence polarization is an excellent method for high throughput binding assays.

Absorption and emission spectra of BDP TMR

Absorption and emission spectra of BDP TMR

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AF 343 (Coumarin)-X-azide

AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.

General properties

Appearance: purple solid
Mass spec M+ increment: 380.2
Molecular weight: 495.28
CAS number: 485397-12-4
Molecular formula: C25H24BF2N3O5
Solubility: good in most organic solvents (DMF, DMSO, DCM, acetone, etc.)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 542
ε, L⋅mol−1⋅cm−1: 55000
Emission maximum, nm: 574
Fluorescence quantum yield: 0.64
CF260: 0.16
CF280: 0.16

Product citations

  1. Nascimento, A.; Pedro, M.N.S.; Pinto, I.F.; Aires-Barros, M.R.; Azevedo, A.M. Microfluidics as a high-throughput solution for chromatographic process development – The complexity of multimodal chromatography used as a proof of concept. Journal of Chromatography A, 2021, 1658, 462618. doi: 10.1016/j.chroma.2021.462618
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