FAM NHS ester, 6-isomer

FAM (Fluorescein) NHS esters Protein labeling Fluorescence polarization
Cat. # Quantity Price Lead time
15120 1 mg –   in stock
25120 5 mg $110 in stock
45120 25 mg $145 in stock
55120 50 mg $260 in stock
65120 100 mg $470 in stock
85120 1 g please inquire in stock
95120 5 g please inquire in stock
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Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.

Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.

This product contains the pure 6-isomer of fluorescein.

FAM NHS ester, 6-isomer
FAM NHS ester, 6-isomer
FAM NHS ester, 6-isomer
6-FAM NHS ester structure
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General properties

Appearance: yellow crystalline solid
Mass spec M+ increment: 358.0
Molecular weight: 473.39
CAS number: 92557-81-8
Molecular formula: C25NO9H15
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Chen, Z.; Zakrzewska, S.; Hajare, H.S.; Du Bois, J.; Minor, D.L. Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry. STAR Protocols, 2024, 5(1), 102792. doi: 10.1016/j.xpro.2023.102792
  2. Kang, N.-W.; Seo, Y.A.; Jackson, K.J.; Jang, K.; Song, E.; Han, U.; Chen, F.; Heilshorn, S.C.; Myung, D. Photoactivated growth factor release from bio-orthogonally crosslinked hydrogels for the regeneration of corneal defects. Bioactive Materials, 2024, 40, 417-429. doi: 10.1016/j.bioactmat.2024.05.045
  3. Haynes, J.R.; Whitmore, C.A.; Behof, W.J.; Landman, C.A.; Ong, H.H.; Feld, A.P.; Suero, I.C.; Greer, C.B.; Gore, J.C.; Wijesinghe, P.; Matsubara, J.A.; Wadzinski, B.E.; Spiller, B.W.; Pham, W. Targeting soluble amyloid-beta oligomers with a novel nanobody. Scientific Reports, 2024, 14, 16086. doi: 10.1038/s41598-024-66970-6
  4. Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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