sulfo-Cyanine5 bis-NHS ester

Cat. # Quantity Price Lead time
133D0 1 mg $125 in stock
233D0 5 mg $325 in stock
433D0 25 mg $850 in stock
533D0 50 mg $1490 in stock
633D0 100 mg $1990 in stock
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sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5® bis-NHS ester.

The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.

Absorption and emission spectra of sulfo-Cyanine5

Absorption and emission spectra of sulfo-Cyanine5

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Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

General properties

Appearance: dark blue powder
Molecular weight: 975.14
Molecular formula: C45H51KN4O14S2
Solubility: good in DMF, DMSO, water
Quality control: NMR 1H, HPLC-MS (895%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Michalski, J.; Kalwarczyk, T.; Kwapiszewska, K.; Enderlein, J.; Poniewierski, A.; Karpińska, A.; Kucharska, K.; Hołyst, R. Rotational and translational diffusion of biomolecules in complex liquids and HeLa cells. Soft Matter, 2024, 20, 5810-5821. doi: 10.1039/d4sm00422a
  2. Pettersson, J.R.; Lanni, F.; Rule, G.S. Dual lifetimes for complexes between Glutathione-S-transferase (hGSTA1-1) and product-like ligands detected by single-molecule fluorescence imaging. Biochemistry, 2017, 56(31), 4073–4083. doi: 10.1021/acs.biochem.7b00030
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