sulfo-Cyanine5 bis-NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133D0 | 1 mg | $125 | in stock | |
| 233D0 | 5 mg | $325 | in stock | |
| 433D0 | 25 mg |
$850
|
in stock | |
| 533D0 | 50 mg |
$1490
|
in stock | |
| 633D0 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5® bis-NHS ester.
The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.
Absorption and emission spectra of sulfo-Cyanine5
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AF 343 (Coumarin)-X-NHS ester
AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.sulfo-Cyanine3 NHS ester
Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.ROX reference dye for qPCR
ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 975.14 |
| Molecular formula: | C45H51KN4O14S2 |
| Solubility: | good in DMF, DMSO, water |
| Quality control: | NMR 1H, HPLC-MS (895%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Michalski, J.; Kalwarczyk, T.; Kwapiszewska, K.; Enderlein, J.; Poniewierski, A.; Karpińska, A.; Kucharska, K.; Hołyst, R. Rotational and translational diffusion of biomolecules in complex liquids and HeLa cells. Soft Matter, 2024, 20, 5810-5821. doi: 10.1039/d4sm00422a
- Pettersson, J.R.; Lanni, F.; Rule, G.S. Dual lifetimes for complexes between Glutathione-S-transferase (hGSTA1-1) and product-like ligands detected by single-molecule fluorescence imaging. Biochemistry, 2017, 56(31), 4073–4083. doi: 10.1021/acs.biochem.7b00030
Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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