Biotin-11-dCTP
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2715-250nmol | 250 nmol | $140 | in stock | |
| 2715-500nmol | 500 nmol | $235 | in stock | |
| 2715-1umol | 1 umol | $360 | in stock | |
| 2715-5umol | 5 umol | please inquire | in stock |
Biotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP.
Biotin-11-dCTP is a substrate for Klenow fragment, T4 and Taq DNA-polymerases, reverse transcriptase, and terminal transferase. Biotinylated DNA can be detected using alkaline phosphatase-conjugated avidin/streptavidin or relevant fluorescent-labeled antibodies.
Biotin-labeled DNA/cDNA probes can be used for fluorescent hybridization in situ or microarray gene profiling.
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A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).General properties
| Appearance: | colorless solid |
| Molecular weight: | 877.48 |
| Molecular formula: | C28H41Li3N7O16P3S |
| IUPAC name: | ((2R,3S,5R)-5-(4-amino-2-oxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Product citations
- Feng, X.; Cui, X.; Zhang, L.-S.; Ye, C.; Wang, P.; Zhong, Y.; Wu, T.; Zheng, Z.; He, C. Sequencing of N6-Methyl-Deoxyadenosine at Single-Base Resolution across the Mammalian Genome. Molecular Cell, 2024, 84(3), 596-610.e6. doi: 10.1016/j.molcel.2023.12.021
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